RESEARCH ARTICLE
Dentinal Tubule Disinfection with Propolis & Two Extracts of Azadirachta indica Against Candida albicans Biofilm Formed on Tooth Substrate
Dakshita Joy Sinha1, Paridhi Garg1, *, Anurag Verma2, Vibha Malik3, Edgar Richard Maccune3, Agrima Vasudeva1
Article Information
Identifiers and Pagination:
Year: 2015Volume: 9
First Page: 369
Last Page: 374
Publisher ID: TODENTJ-9-369
DOI: 10.2174/1874210601509010369
Article History:
Received Date: 05/12/2014Revision Received Date: 26/08/2015
Acceptance Date: 13/09/2015
Electronic publication date: 12/11/2015
Collection year: 2015

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
Abstract
Aim:
This study evaluates the disinfection of dentinal tubules using Propolis, Azadirachta indica (alcoholic and aqueous extracts), 2% chlorhexidine gel and calcium hydroxide against Candida albicans biofilm formed on tooth substrate.
Materials & Method:
One hundred and five human teeth were infected with Candida albicans for 2 days. Samples were divided into 7 groups. Group I- Propolis, Group II- Alcoholic extract of Azadirachta indica, Group III- Aqueous extract of Azadirachta indica, Group IV- 2% Chlorhexidine, Group V- Calcium hydroxide, Group VI- Ethanol and Group VII- Saline (negative control). At the end of 1,3 and 5 days, the antimicrobial efficacy of medicaments against Candida albicans was assessed at the depths of 200 µm and 400 µm.
Results:
The overall percentage inhibition of fungal growth (at 200 µm and 400 µm depth) was 99.2% with 2% chlorhexidine gel. There was no statistical difference between propolis, alcoholic extract of Azadirachta indica (neem) and 2% chlorhexidine.
Conclusion:
Propolis and alcoholic extract of Azadirachta indica performed equally well as that of 2% Chlorhexidine.