Microstructural Observation with MicroCT and Histological Analysis of Human Alveolar Bone Biopsy from a Planned Implant Site: A Case Report

Emi Yamashita-Mikami1, Mikako Tanaka 1, *, Naoki Sakurai 1, Kazuho Yamada 2, Hayato Ohshima 3, Shuichi Nomura 1, Sadakazu Ejiri 4
1 Division of Comprehensive Prosthodontics, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan
2 Oral Implant Clinic, Niigata University Medical and Dental Hospital, Niigata, Japan
3 Division of Anatomy and Cell Biology of the Hard Tissue, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan
4 Department of Oral Anatomy, Asahi University School of Dentistry, Gifu, Japan

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© Yamashita-Mikami et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Division of Comprehensive Prosthodontics, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, 2-5274 Gakkocho-dori, Chuo-ku, Niigata 951-8514, Japan; Tel: +81-25-227-2892; Fax: +81-25-229-3454; E-mail:


The subject was a 53-year-old male. An alveolar bone sample was obtained from the site of the lower left first molar, before dental implant placement. Although the details of the trabecular structure were not visible with conventional computed tomography, micro-computed tomography (microCT) three-dimensional images of the alveolar bone biopsy sample showed several plate-like trabeculae extending from the lingual cortical bone. Histological observations of the bone sample revealed trabeculae, cuboidal osteoblasts, osteoclasts and hematopoietic cells existing in the bone tissue at the implantation site. Bone metabolic markers and calcaneal bone density were all within normal ranges, indicating no acceleration of the patient’s bone metabolism.

Using microCT, and histological and histomorphometrical techniques, a great deal of valuable information about the bone tissue was obtained from a biopsy sample extracted from the patient’s planned implant site.

Keywords: Bone biopsy, bone micro-structure, dental implant, histomorphometry, human alveolar bone, microCT.