Salivary 8-OHdG Induction by Physical Exercise Training Under Food Restriction

Yoko Yoshino a, *, Yoichi Nakagawa b
a Department of Nutrition and Dietetics, Kamakura Women's University, 6-1-3 Ohfuna, Kamakura, Kanagawa 247-8512, Japan
b Dry mouth Clinic, Tsurumi University Dental Hospital, and Department of Oral and Maxillofacial Surgery, Tsurumi University School of Dental Medicine, Yokohama, Japan

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© Yoshino and Nakagawa; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Department of Nutrition and Dietetics, Kamakura Women's University, 6-1-3, Ohfuna, Kamakura city, Kanagawa 247-8512, Japan; Tel: +467 44 2111; Fax: +467 44 7131; E-mail:



We previously reported that physical exercise under food restriction induced alterations in saliva secretion, including a decrease in salivary kallikrein activity, while exercise training alone did not affect the overall saliva content. The objective of the present study was to examine the involvement of oxidative stress in alterations of salivary secretion due to physical exercise under food restriction.


Male ICR mice at 32 weeks of age weighing from 39 to 50 g were divided into three groups: an exercise group with food restriction (EXP), an exercise group without food restriction (EXA) and a control group (CTL). The EXP group was fed the same amount of diet as the CTL group (pair-feeding). The EXP and EXA groups had access to a “voluntary running wheel” for exercise. The pilocarpine-stimulated whole saliva was collected from the oral cavity by micro-pipette over 15 min for 12 weeks after the beginning of the experiment. The salivary and serum 8-Hydroxy-2’-deoxyguanosine (8-OHdG) were determined.


The salivary 8-OHdG levels in the EXP group were significantly higher than in the CTL and EXA groups (p<0.01).


The alterations of salivary secretion in mice undergoing chronic exercise training under a food-restricted diet may occur as a result of oxidative stress.

Key Words: Saliva, 8-OHdG, Oxidative stress, Exercise training, Food restriction.