To Compare the Microleakage Among Experimental Adhesives Containing Nanoclay Fillers after the Storages of 24 Hours and 6 Months

Seyed Mostafa Mousavinasab*, 1, Mohammad Atai 2, Bagher Alavi 3
1 Isfahan University of Medical Sciences, Dental School, Department of Operative Dentistry and Torabinejad Research Centre, Isfahan, Iran
2 Iran Polymer and Petrochemical Institute (IPPI), Tehran, Iran
3 Operative Dentistry Department, Dental School, Isfahan University of Medical Sciences, Isfahan, Iran

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© Mousavinasab et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Isfahan University of Medical Sciences, Iran; Tel:0098 311 7922849; Fax: 0098 311 6687080; E-mail:,



To compare the microleakage among experimental adhesives containing nanoclay fillers after the storages of 24 hours and 6 months.

Materials and Methods:

Class V cavities were prepared on extracted human molars with the occlusal margins located in enamel and the cervical margins in cementum. Phosphoric acid was applied to the enamel and dentin margins.Subsequently, the cavities were treated using four groups of experimental adhesive systems and restored with a resin composite. Adper Single Bond® was used as control group. After 24- hour and 6- month storages, the samples were subjected to thermocycling shocks and then immersed in silver nitrate as well as developer solution and finally evaluated for leakage. The data were analyzed using SPSS software.


Based on Kruskal –Wallis test, significant differences were found between groups regarding microleakage. The Mann- Whitney test showed that Leakage was significantly lower in Adper Single Bond® compared to the other groups in dentinal margins after 24 hours and 6 months and in enamel margins after 6 months. The Wilcoxon Signed Ranks test showed that the enamel leakage in experimental adhesives was significantly lower than dentinal leakage after 24 hours as well as enamel leakage in Adper Single Bond and adhesive with 0.5% PMAA-g-nanoclay was significantly lower than dentinal margins after storage period of 6 months.


All the experimental adhesives were effective in reducing enamel leakage after 24 hours, but were not effective in reducing dentinal leakage after 24 hours as well as in enamel and dentinal leakage after a 6-month storage. No improvement was observed in the microleakage in dentin in both short (24 hrs) and long times (6 months).

The high microleakage in the adhesives is probably attributed to the high concentration of HEMA in the recipe of the bonding agent.

Key Word: Dentin bonding, microleakage, nanoclay, nanoparticle containing adhesives.