In vivo Spectrophotometric Assessment of the Tooth Whitening Effectiveness of Nite White 10% with Amorphous Calcium Phosphate, Potassium Nitrate and Fluoride, Over a 6-month Period

Sias R Grobler*, Abdul Majeed, Mohamad H Moola, Roelof J Rossouw , Theuns van Wyk Kotze
Oral & Dental Research Institute, Faculty of Dentistry, Tygerberg, University of the Western Cape, Cape Town, South Africa

Article Metrics

CrossRef Citations:
Total Statistics:

Full-Text HTML Views: 366
Abstract HTML Views: 160
PDF Downloads: 65
Total Views/Downloads: 591
Unique Statistics:

Full-Text HTML Views: 175
Abstract HTML Views: 123
PDF Downloads: 60
Total Views/Downloads: 358

Creative Commons License
© Grobler et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Private Bag X1, Oral and Dental Research Institute, Faculty of Dentistry, University of the Western Cape, Tygerberg 7505, Cape Town, South Africa; Tel: +27 21 937 3023; Fax: +27 21 937 3025; E-mail:


To clinically evaluate the effectiveness of Nite White 10% carbamide peroxide with amorphous calcium phosphate, potassium nitrate and fluoride over a 6-month follow-up period. Nite White was applied nightly for 14 days, according to the manufacturer’s instructions. The color of teeth 11 and 21 of twenty one subjects was measured with a spectrophotometer (L*; a*; b*). Subjects were instructed to take note of any tooth sensitivity and gingival irritation. For all three components (L*, a* and b*) statistical significant differences (p<0.05) in the values between base-line (pre-bleaching) and; after treatment, after 1-month, after 3-months as well as after 6-months were found (Wilcoxon Signed Rank Sum Test). Significant differences were also found amongst the ΔE*ab (0-14days) values and; ΔE*ab (0-1 month), ΔE*ab (0-3 months) and ΔE*ab (0-6 months). However, no significant differences were found between pairs of ΔE*ab (0-1 month), ΔE*ab (0-3 months) and ΔE*ab (0-6months). The decrease in ΔE*ab was the highest after 1 month (~30%). The highest decrease in L* was about 58% after 1 month. Over the 14-day treatment period tooth sensitivity was 24.5%. Conclusions: Nite White ACP demonstrated significant tooth-whitening (unit increase = 5.29) with a low tooth sensitivity (25%) probably due to the presence of amorphous calcium phosphate, potassium nitrate, and fluoride. The whitening effect decreased the most after one month and then maintained well even after a 6 month period (units 3.89).

Clinical implications

The product is a good tooth whitener with a color increase of about 5 units accompanied with a low sensitivity.

Keywords: Clinical study, tooth whitener, Nite White 10% carbamide peroxide (ACP), spectrophotometer.