RESEARCH ARTICLE


Applications of Surface-Enhanced Laser Desorption/Ionization Time-Of-Flight (SELDI-TOF) Mass Spectrometry in Defining Salivary Proteomic Profiles



Sandra K Al-Tarawneh1, Sompop Bencharit1, 2, *
1 Department of Prosthodontics, School of Dentistry, University of North Carolina, Chapel Hill, NC, USA
2 Department of Pharmacology, School of Medicine, and the Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC, USA


Article Metrics

CrossRef Citations:
0
Total Statistics:

Full-Text HTML Views: 2093
Abstract HTML Views: 1186
PDF Downloads: 365
Total Views/Downloads: 3644
Unique Statistics:

Full-Text HTML Views: 1086
Abstract HTML Views: 682
PDF Downloads: 242
Total Views/Downloads: 2010



Creative Commons License
© Al-Tarawneh and Bencharit; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the CB #7450, Department of Prosthodontics, School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7450, USA; E-mail: sompop_bencharit@dentistry.unc.edu


Abstract

Recent advancement in mass spectrometry leads us to a new era of proteomic analysis. Human saliva can be easily collected; however, the complexity of the salivary proteome in the past prevented the use of saliva for proteomic analysis. Here we review the development of proteomic analyses for human saliva and focus on the use of a new mass spectrometric technology known as surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF). SELDI-TOF, a modification of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF), combines the precision of mass spectrometry and the high-through-put nature of protein arrays known as Protein Chips. This technology shows a promising future for salivary proteomic analysis in monitoring treatments and diseases, as well as novel biomarker discovery.

Key Words: Proteomics, mass spectrometry, biomarker, biomarker discovery, proteomic profiling, protein expression, SELDI, SELDI-TOF, protein chips.