Effects of Aging and Cyclosporin A on Collagen Turnover in Human Gingiva

N Gagliano1, *, F Costa1, G.M Tartaglia1, L Pettinari1, F Grizzi2, C Sforza1, N Portinaro3, M Gioia1, G Annoni4
1 Department of Human Morphology and Biomedical Sciences “Città Studi”, EML-Extracellular Matrix Laboratory, Università degli Studi di Milano, School of Medicine, Milan, Italy
2 Laboratories of Quantitative Medicine, Istituto Clinico Humanitas IRCCS, Rozzano, Milan, Italy
3 Department of Pediatric Orthopaedic Surgery, Istituto Clinico Humanitas, Rozzano, Milan, Italy
4 Department of Internal Medicine and Prevention and Geriatric Unit, S.Gerardo Hospital, University of Milano-Bicocca, Milan, Italy

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© Gagliano et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the University of Milan, Department of Human Morphology and Biomedical Sciences “Città Studi”– LITA, Extracellular Matrix Laboratory, Via Fratelli Cervi 93, 20090 Segrate – Milano, Italy; Tel: +39 02 50330462; Fax: +39 02 50330452; E-mail:



We aimed at characterizing the aging gingiva analyzing: i) collagen content and turnover in human gingival tissues and fibroblasts obtained from healthy young and aging subjects. ii) the effect of cyclosporin A administration in human cultured gingival fibroblasts obtained from aging compared to young subjects.


Morphological analysis was performed on haematoxylin-eosin and Sirius red stained paraffin-embedded gingival biopsies from young and aging healthy subjects. The expression of the main genes and proteins involved in collagen turnover were determined by real time PCR, dot blot and SDS-zymography on cultured young and aging gingival fibroblasts, and after cyclosporin A administration.


Our results suggest that in healthy aged people, gingival connective tissue is characterized by a similar collagen content and turnover. Collagen turnover pathways are similarly affected by cyclosporin A treatment in young and aging gingival fibroblasts.


Cyclosporin A administration affects gingival collagen turnover pathways in young and aging fibroblasts at the same extent, suggesting that during aging cyclosporin A administration is not related to relevant collagen turnover modifications.

Keywords: Aging, gingiva, collagen turnover, matrix metalloproteinases, SPARC, cyclosporin A, gingival overgrowth.