An In vivo Model for Short-Term Evaluation of the Implantation Effects of Biomolecules or Stem Cells in the Dental Pulp

Sally Lacerda-Pinheiro1, Arnaud Marchadier2, Patricio Donãs 3, Dominique Septier3, Laurent Benhamou2, Odile Kellermann1, Michel Goldberg3 , *, Anne Poliard1
1 Laboratoire de Différenciation Cellulaire, Cellules Souches et Prions, IFR- 2937 CNRS, Villejuif, France
2 Laboratoire de Caractérisation du Tissue Osseux par Imagerie, INSERM U658, Orléans, France
3 Laboratoire Régénération et Réparation des Tissus Cranio-faciaux, EA2496, Faculté de Chirurgie Dentaire, Université Paris - Descartes, Montrouge, France

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© Lacerda-Pinheiro et al.; Licensee Bentham Open

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited.

* Address correspondence to this author at the Faculté de Chirurgie Dentaire, 1 rue Maurice Arnoux , 92120, Montrouge, France; Tel: +33 158076808; Fax: +33 158076806; E-mail:


The continuously growing rodent incisor is a widely used model to investigate odontogenesis and mineralized tissue formation. This study focused on evaluating the mouse mandibular incisor as an experimental biological tool for analyzing in vivo the capacity of odontoblast-like progenitors or bioactive molecules to contribute to reparative dentinogenesis. We describe here a surgical procedure allowing direct access to the forming part of the incisor dental pulp Amelogenin peptide A+4 adsorbed on agarose beads, or dental pulp progenitor cells were implanted in the pulp following this procedure. After 10 days A+4 induced the formation of an osteodentin occluding almost the totality of the pulp compartment. Implantation of progenitor cells leads to formation of islets of osteodentin-like structures located centrally in the pulp. These pilot studies validate the incisor as an experimental model to test the capacity of progenitor cells or bioactive molecules to induce the formation of reparative dentin.

Key Words: Mouse incisor, dental pulp progenitor cells, amelogenin, experimental model.