The continuously growing rodent incisor is a widely used model to investigate odontogenesis and mineralized tissue formation. This study focused on evaluating the mouse mandibular incisor as an experimental biological tool for analyzing in vivo the capacity of odontoblast-like progenitors or bioactive molecules to contribute to reparative dentinogenesis. We describe here a surgical procedure allowing direct access to the forming part of the incisor dental pulp Amelogenin peptide A+4 adsorbed on agarose beads, or dental pulp progenitor cells were implanted in the pulp following this procedure. After 10 days A+4 induced the formation of an osteodentin occluding almost the totality of the pulp compartment. Implantation of progenitor cells leads to formation of islets of osteodentin-like structures located centrally in the pulp. These pilot studies validate the incisor as an experimental model to test the capacity of progenitor cells or bioactive molecules to induce the formation of reparative dentin.
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We thank Arthur Veis for his generous gift A+4. Sally Lacerda-Pinheiro was supported by Coordenação de Aperfeiçoamento de Pessoal de Nivel Superior (CAPES), Brazil.This research was supported by Fondation de lAvenir,lsInstitut Français pour la Recherche Odontologique (IFRO)and an Inserm “Somatic Stem Cells Research Grant”(N.CS0307)
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