Rodent Gingival Tissue Culture in an Aging Experimental Model: A Pilot Study

Arrum Mutiara1, Benso Sulijaya2, *, Sri Lelyati C. Masulili2, Boy M. Bachtiar3, Ines A. Sumbayak1, Fatimah Maria Tadjoedin2, Permana Wati4, Devi Kartika4
1 Periodontology Specialist Program, Department of Periodontology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
2 Department of Periodontology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
3 Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
4 Bimana Animal Facility, Bogor Agriculture Institute, Bogor, West Java, Indonesia

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© 2022 Mutiara et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Periodontology, Faculty of Dentistry, Universitas Indonesia, Jl. Salemba Raya No. 4, Jakarta Pusat, 10430, Indonesia; Email:,



Gingiva acts as a barrier to prevent further invasion of pathogens in periodontitis. The gingival structure consists of epithelial tissue and connective tissue. As the aging process continues, there are several changes in the periodontium. Previous studies have tried to investigate the complex interaction between the host immune system and bacteria by using animal models, especially rodents.


The aim of the study was to evaluate the effectiveness of collecting gingival tissue from the palate and retromolar pad.

Materials and Methods:

The aging experimental model had two age categories of male rodents of 18 and 58 weeks. Tissue was collected from the mandible retromolar pad and palate with full-thickness excision. Tissues were transferred to a complete medium at 4°C. Gingival tissue was cultured in a 37°C culture incubator at 5% CO2. Tissue proliferation was observed on the first, third, and fifth days using the hemocytometer. The cell metabolism rate between the two age categories was checked using the MTT Assay. Two-way ANOVA was used for statistical analysis.


Gingival tissues obtained from the experimental models of two age categories were alive, and proliferation was observed. The old rodent group showed no significant result in terms of cell morphology on the first vs. third day (p>0.05), but significant results were found on the first vs. fifth day and third day vs. the fifth day (p<0.05). The young rodent group showed the most significant morphology changes between days. In both young and old categories, no significant difference was observed in the cell metabolism.


Rodent gingival tissue collection from the retromolar pad and palate was found suitable for tissue culture in the aging experimental study.

Keywords: Gingiva, Tissue culture, Animal study, Gingiva epithelial, Barrier function, Aging.