Efficacy of MTA Modified by Nanosilver for the Prevention of Coronal Leakage

Shifteh Nasri1
, Farzaneh Afkhami2, *

1 Private practice, Tehran, Iran
2 Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, International Campus, Tehran, Iran

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© 2021 Nasri and Afkhami

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Mahan St. Zam Zam St. Navab Highway, P.O. code: 1894787545, Tehran, Iran; Tel:(+98 21) 55851131; E-mails:,



Numerous materials have been introduced as coronal barriers, however, they have shown various degrees of microleakage. Therefore, attempts are undertaken to introduce more reliable materials with the potential to provide a long-term coronal seal.


This in vitro study aimed to assess the efficacy of gray ProRoot Mineral Trioxide Aggregate (MTA) modified by a suspension of silver nanoparticles (AgNPs) as an orifice plug using a bacterial leakage method.


40 extracted human teeth were prepared and filled (except for the negative control group) using the lateral compaction technique. The coronal 2 mm of the root filling was removed in the experimental groups. The teeth were randomly divided into two experimental (n = 15) and two control (n = 5) groups. In the experimental group 1 (MTA group) and the experimental group 2 (Ag-MTA group), MTA modified by a suspension of AgNPs was used as an orifice plug. In the negative control group, the entire root surfaces were covered with two layers of nail varnish. In the positive control group, the root canals were filled with a single gutta-percha cone without a sealer and no orifice plug. Bacterial leakage was assessed using a two-chamber system. The teeth were incubated at 37°C and 100% humidity for 120 days, and human saliva was added to the samples every 3 days. Bacterial microleakage was assessed by daily monitoring and observating the turbidity of the Brain Heart Infusion (BHI) broth. The data were analyzed using the log-rank test.


All positive controls showed contamination after 5 days. None of the negative controls were contaminated during the experiment. The Ag-MTA group demonstrated a significantly better coronal seal than the MTA group (p = 0.031).


Gray ProRoot MTA modified by AgNPs has the potential for being used as an orifice plug in endodontically treated teeth.

Keywords: Bacterial leakage, Coronal microleakage, Coronal seal, Orifice seal, ProRoot MTA, Human teeth.