RESEARCH ARTICLE
The Evaluation of Osteoblastic Cell Behavior on Treated Titanium Surface
Maria A. S. De Souza Alencar1, Elizabeth F. Martinez1, Fábio C. Figueiredo1, André R. De Lima e Silva1, José E. Protazio1, Maicon Bertamoni1, Daiane C. Peruzzo1, Marcelo H. Napimoga1, *
Article Information
Identifiers and Pagination:
Year: 2020Volume: 14
First Page: 1
Last Page: 6
Publisher ID: TODENTJ-14-1
DOI: 10.2174/1874210602014010001
Article History:
Received Date: 15/09/2019Revision Received Date: 13/11/2019
Acceptance Date: 19/12/2019
Electronic publication date: 14/02/2020
Collection year: 2019
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Background:
There are several potential advantages in optimizing the initial events of osseointegration in the benefit of clinical outcome.
Objective:
The objective of the present study was to evaluate the behavior of osteoblastic cells on surfaces treated by double acid etching using HNO3 and H2SO4.
Methods:
Commercially pure titanium (grade 4) discs measuring 6 mm in diameter and 2 mm in thickness were used. The discs were divided into two groups: machined group and double acid-etched discs (HNO3 and H2SO4). Surface characteristics were assessed using Scanning Electron Microscopy. Pre-osteoblastic MC3T3-E1 cells were used for cell culture on the tested surfaces to assess proliferation, viability (MTT), as well as secretion (ELISA) and cytoplasmic expression (Western blot) of type I collagen.
Results:
The data obtained were analyzed using t-test or two-way ANOVA followed by Bonferroni’s test at 95% significance. The titanium surfaces showed average roughness values for the machined and treated surfaces of 0.29 and 1.16, respectively (p<0.05). An increase in cell proliferation was observed, which was corroborated by the viability assay. Both type I collagen secretion and intracellular expression were higher on the double acid-etched surface compared to the machine surfaces (p<0.05).
Conclusion:
Implant surfaces treated by double acid etching positively affected the early events of the interaction between titanium and osteoblastic cells, suggesting optimization of osseintegration.
