RESEARCH ARTICLE


The Evaluation of Osteoblastic Cell Behavior on Treated Titanium Surface



Maria A. S. De Souza Alencar1, Elizabeth F. Martinez1, Fábio C. Figueiredo1, André R. De Lima e Silva1, José E. Protazio1, Maicon Bertamoni1, Daiane C. Peruzzo1, Marcelo H. Napimoga1, *
1 Laboratory of Immunology and Molecular Biology, São Leopoldo Mandic Institute and Research Center, Campinas, SP, Brazil


Article Metrics

CrossRef Citations:
0
Total Statistics:

Full-Text HTML Views: 1166
Abstract HTML Views: 119
PDF Downloads: 0
Total Views/Downloads: 1285
Unique Statistics:

Full-Text HTML Views: 536
Abstract HTML Views: 74
PDF Downloads: 0
Total Views/Downloads: 610



Creative Commons License
© 2020 Alencar et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Laboratory of Immunology and Molecular Biology, São Leopoldo Mandic Institute and Research Center, R. José Rocha Junqueira, 13 - 13045-755-Campinas, SP, Brazil; Tel: +55 19 3211-3627; Fax: +55 19 3211-3712; E-mails: marcelo.napimoga@gmail.com or marcelo.napimoga@slmandic.edu.br


Abstract

Background:

There are several potential advantages in optimizing the initial events of osseointegration in the benefit of clinical outcome.

Objective:

The objective of the present study was to evaluate the behavior of osteoblastic cells on surfaces treated by double acid etching using HNO3 and H2SO4.

Methods:

Commercially pure titanium (grade 4) discs measuring 6 mm in diameter and 2 mm in thickness were used. The discs were divided into two groups: machined group and double acid-etched discs (HNO3 and H2SO4). Surface characteristics were assessed using Scanning Electron Microscopy. Pre-osteoblastic MC3T3-E1 cells were used for cell culture on the tested surfaces to assess proliferation, viability (MTT), as well as secretion (ELISA) and cytoplasmic expression (Western blot) of type I collagen.

Results:

The data obtained were analyzed using t-test or two-way ANOVA followed by Bonferroni’s test at 95% significance. The titanium surfaces showed average roughness values for the machined and treated surfaces of 0.29 and 1.16, respectively (p<0.05). An increase in cell proliferation was observed, which was corroborated by the viability assay. Both type I collagen secretion and intracellular expression were higher on the double acid-etched surface compared to the machine surfaces (p<0.05).

Conclusion:

Implant surfaces treated by double acid etching positively affected the early events of the interaction between titanium and osteoblastic cells, suggesting optimization of osseintegration.

Keywords: Dental implant, Osseointegration, Surface roughness, Osteoblastic cell, Titanium, HNO3 and H2SO4.