Dentin Cleaning Ability of an Amazon Bioactive: Evaluation by Scanning Electron Microscopy

Maria Fulgência C.L. Bandeira1, *, Geisy R. Lima1, Patrícia P. Lopes1, Carina Toda1, Gisely N. Venâncio1, Greiciane A. Lima1, Marne C. de Vasconcellos1, Leandro M. Martins1, Fâbio C. Sampaio2, Nikeila C. de Oliveira Conde1
1 School of Dentistry, Federal University of Amazonas, Manaus, AM, Brazil
2 School of Dentistry, Federal University of Paraíba, João Pessoa-PB, Brazil

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© Bandeira et al:, Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (, which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the School of Dentistry, Federal University of Amazonas, Manaus, AM, Brazil; E-mail:


The role of dentin cleaning is to remove debris that may impair adaptation and marginal sealing, quantitatively reducing microorganisms. The aim of this study was to investigate through scanning electron microscopy (SEM) the morphology of the dentin surface, cut and treated with copaiba oil emulsions (CO) and suspension of ethanol extract of propolis (EP). Twenty four upper pre-molars teeth, divided into eight groups (n=3), were used: G1: no cleaning, G2: air/water spray, G3: 10% CO, G4: 10% CO + A, G5: 30% CO, G6: 30% CO + A, G7: 1% EP, G8: 2% Chlorhexidine. The specimens were dentin discs (1 mm Ø). The SEM photomicrographs were classified and the results were: G1 - Debris dentin on the entire image / countless microorganisms, G2 and G7 - 50-100 debris / countless microorganisms and G3, G4, G5, G6 and G8 - 0-50 debris / countable microorganisms (50-100 colonies).


The present results suggest that copaiba oil emulsions (CO) and suspension of ethanol extract of propolis (EP) have feasibility to be used as bioactive dental cleaning agents.

Keywords: Copaifera, dentin, integrative and complementary practices, phytotherapy, propolis, scanning electron microscopy.