Effects of Citric Acid and Desensitizing Agent Application on Nonfluorosed and Fluorosed Dentin: An In Vitro Sem Study

Mahajan Neha , Laxman K Vandana*
Department of Periodontics, College of Dental Sciences, Davangere, Karnataka, India

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© Neha and Vandana; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Department of Periodontics, College of Dental Sciences Davangere, Karnataka, India; Tel: +91-8192-30432 / 31285; E-mail:


Fluorosis is one of the factors which bring about mineralisation changes in a dentinal structure leading to dentin. The purpose of the present study was to compare and evaluate the dentinal tubular changes in fluorosed and nonfluorosed teeth subsequent to the application of citric acid,strontium acetate based sodium fluoride (SAF) using scanning electron microscopy (SEM). Dentin specimens from healthy fluorosed and nonfluorosed teeth were included in the study. Each of them was grouped into acid treated and SAF treatment groups. Using SEM, the photomicrographs (3500x) of dentin specimens were evaluated. Results showed while there was a significant difference in tubular width of partial occlusion ≤ 25%, being more in fluorosed group compared to nonfluorosed group after application SAF. Application of desensitising agents demonstrated higher number of dentinal tubular occlusion and diameter reduction in nonfluorosed dentin compared to fluorosed dentin. Summary: Root biomodification and desensitising agent procedure brings in definite difference between fluorosed and non-fluorosed dentin specimens.

Keywords: Citric acid, desensitising agent, fluorosed dentin, strontium acetate.