Combination of Root Surface Modification with BMP-2 and Collagen Hydrogel Scaffold Implantation for Periodontal Healing in Beagle Dogs

Akihito Kato 1, Hirofumi Miyaji *, 1, Ryosuke Ishizuka 1, Keisuke Tokunaga 1, Kana Inoue 1, Yuta Kosen 1, Hiroyuki Yokoyama 1, Tsutomu Sugaya 1, Saori Tanaka 1, Ryuji Sakagami 2, Masamitsu Kawanami 1
1 Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan
2 Section of Periodontology, Department of Odontology, Fukuoka Dental College, Fukuoka, Japan

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© Kato et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Department of Periodon-tology and Endodontology, Hokkaido University Graduate School of Dental Medicine, N13 W7 Kita-ku, Sapporo 060-8586, Japan; Tel: +81 11 706 4266; Fax: +81 11 706 4334; E-mail:


Objective : Biomodification of the root surface plays a major role in periodontal wound healing. Root surface modification with bone morphogenetic protein (BMP) stimulates bone and cementum-like tissue formation; however, severe ankylosis is simultaneously observed. Bio-safe collagen hydrogel scaffolds may therefore be useful for supplying periodontal ligament cells and preventing ankylosis. We examined the effects of BMP modification in conjunction with collagen hydrogel scaffold implantation on periodontal wound healing in dogs. Material and Methods: The collagen hydrogel scaffold was composed of type I collagen sponge and collagen hydrogel. One-wall infrabony defects (5 mm in depth, 3 mm in width) were surgically created in six beagle dogs. In the BMP/Col group, BMP-2 was applied to the root surface (loading dose; 1 µg/µl), and the defects were filled with collagen hydrogel scaffold. In the BMP or Col group, BMP-2 coating or scaffold implantation was performed. Histometric parameters were evaluated at 4 weeks after surgery. Results: Single use of BMP stimulated formation of alveolar bone and ankylosis. In contrast, the BMP/Col group frequently enhanced reconstruction of periodontal attachment including cementum-like tissue, periodontal ligament and alveolar bone. The amount of new periodontal ligament in the BMP/Col group was significantly greater when compared to all other groups. In addition, ankylosis was rarely observed in the BMP/Col group. Conclusion: The combination method using root surface modification with BMP and collagen hydrogel scaffold implantation facilitated the reestablishment of periodontal attachment. BMP-related ankylosis was suppressed by implantation of collagen hydrogel.

Keywords: Ankylosis, bone morphogenetic protein-2, collagen hydrogel, dog, one-wall infrabony defects, periodontal attachment, periodontal wound healing, regenerative scaffold. .