Intracanal Antibiotic Medication for Sustained Root Surface Disinfection– A Laboratory Evaluation

Markus Tobias Winfried Zaruba1, Tilla Filli1, Dan-Krister Rechenberg1, Thomas Thurnheer2, Thomas Attin1, Patrick Roger Schmidlin1, *
1 Clinic of Preventive Dentistry, Periodontology and Cariology, University of Zurich, Switzerland
2 Section for Oral Microbiology and Immunology, Institute of Oral Biology, Center of Dental Medicine, University of Zurich, Switzerland
3 Department of Oral Medicine, Valencia University Medical and Dental School, Spain

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© Zaruba et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the (, which permits unrestricted, noncommercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Clinic of Preventive Dentistry, Periodontology and Cariology, Center of Dental Medicine, University of Zurich, Plattenstrasse 11, 8032 Zurich, Switzerland; Tel: +41 44 634 3934; Fax: +41 44 634 43 08; E-mail:


Purpose: To measurethe release of an antibiotic mixture of ciprofloxacin, cerfuroxim and metronidazole (TreVitaMix, TVM) through human dentine and to assess the growth inhibition of Fusobacterium nucleatum. Material and Methods: Twenty-four extracted human incisors were scaled and endodontically treated. Root canals were either filled with antibiotic tri-mixture (TVM) or with the carrier material alone (propylene glycol, PG) and were coronally and apically sealed with a flowable composite. Transradicular medicament release was spectrophotometrically measured at 277 nm in simulated body fluid for up to 21 days. In a second part, an agar diffusion assay (F. nucleatum) with representative TVM concentrations as determined in the first part was performed to study the growth inhibition. Samples were anaerobical incubated for 48 h and inhibition zones were measured. Results:TVM was spectrophotometrically detectable in the immersion solution and released in decreasing concentrations up to 21 days (222.5 ± 65.2 mg/ml at day 1 and 35.1 ± 15.6 mg/ml at day 21). In addition, inhibition zones were shown in the agar diffusion assay at representative TVM concentrations. The carrier material showed no antibacterial effect. Conlusion: TVM showed the potential to penetrate through dentine and to inhibit bacterial growth. Therefore, it might have the potential to disinfect the outer root surface in perio-endo lesions, but further research is needed to confirm these observations.

Keywords:: Agar diffusion, bacteria, dentine, perio-endo lesion, TreVitaMix.