A Clinical Study of the Effectiveness of a Light Emitting Diode System on Tooth Bleaching

Roche Hayward, Yusuf Osman , Sias R Grobler*
Oral and Dental Research Institute, Faculty of Dentistry, University of the Western Cape, Private Bag X1, Tygerberg 7505, Cape Town, South Africa

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© Hayward et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Oral and Dental Research Institute, Faculty of Dentistry, University of the Western Cape, Private Bag X1, Tygerberg 7505, Cape Town, South Africa; Tel: +27 937 3024; Fax: +27 937 3025; E-mail:


The purpose of this study was to evaluate the efficacy of a Light Emitting Diode (LED) System (BriteWhite) on tooth bleaching. Method: eeth 11 and 21 of twenty one subjects were treated in the chair with a LED light and 44% carbamide peroxide gel, followed by an at-home treatment period of 14 days with 35% carbamide peroxide. The color of the teeth was measured with a spectrophotometer (L*; a*; b*) before treatment (control) and after the above mentioned two stages. The subjects were also instructed to note tooth and gingival sensitivity. Results: For the L* component a statistically significant difference (p<0.05) between base-line and directly after the LED treatment stage (whiter) was found and a complete relapse was found after the 14 day at-home treatment period. The b* component showed statistically significant differences (p<0.05) between base-line and the LED stage (less yellow more blue), with a further significant color improvement after the 14 day treatment period. For the a* component (green to red) no statistically significant change was (p>0.05) found throughout the study (Wilcox on Signed Rank Sum Test). A major color change of 1.8 units (ΔEab) was found after the LED treatment stage with only a further small 0.2 unit improvement after the 14 day at-home treatment stage. Tooth and gingival sensitivity scores were low (below mild) throughout the treatment stages. Conclusion: A major tooth color increase was found after the in-office LED/gel treatment stage and only an insignificant further improvement was noted after the additional 14 day at-home treatment period. Only low tooth and gingival sensitivity scores were found. A slight increase in both the sensitivity and gingival scores after the LED/gel activated stage could be observed. Overall the total color increase was low.

Keywords: BriteWhite system, LED light, spectrophotometer, tooth whitener.


Early in history humans started experimenting with the use of different chemicals to whiten their teeth for aesthetic purposes. However, the first commercial marketing of a 10% carbamide peroxide whitener occurred only in 1989 [1]. Nowadays various treatment modalities are available which include over-the-counter bleaching (self-administered), in-office bleaching (professionally administered) and dentist-supervised take-home bleaching (professionally dispensed). Modern society desires to see the effect of bleaching immediately, resulting in higher concentrations of chemicals used in the composition of the whiteners [2] with different light sources believed to accelerate the bleaching process [3]. These high concentrations should only be applied by qualified people for control and prevention of possible damage to oral soft tissue. Today in-office bleaching mainly uses carbamide peroxide (CP) or hydrogen peroxide (HP) which might be activated by heat or light (with a chemical catalyst) to catalyze the tooth bleaching process [2, 4]. It is believed that most light sources decompose peroxide faster (by increasing the temperature) to form free radicals which whiten teeth [2, 5, 6]. Various light sources are available, for example: light-emitting diodes (LED’s), lasers, halogen lamps and plasma arc lamps (PAC). The most important point is that activation by heat, light or laser should not increase the intrapulpal temperature with more than 5.5 °C [3] to avoid tooth damage.

However, strong controversy surrounds the success of light sources. Some researchers believe that it is effective in the bleaching process, while others believe only certain lights are effective and others reported no effect [4, 6-18]. Gurgan et al., 2010 [12] investigated the effect of 3 different light systems [diode laser, 810 nm on 37% HP; plasma arc lamp, 400-490 nm on 35% HP; light emitting diode lamp on 38% HP] and found that the diode laser system gave the best tooth whitening (∆E*ab 5.69) and the least tooth sensitivity (on a 1-10 scale; 0.59 against approximately 3) as measured with a spectrophotometer. However, these differences were small and shade guide measurements gave no significant differences between the lights mentioned. Recently (2012) it was reported [16] that a halogen light is more effective than a laser light, while Hahn [17] (2012) could not find an improvement in tooth whitening as a result of LED or laser light treatments. He also reported no positive tooth color effect because of light treatment with an increase in tooth sensitivity. Hein (2003) [11] reported no difference in the whitening effect of bleaching gels [25%-35% (HP)] with or without 3 different lights. They concluded that the proprietary chemicals added to the bleaching gels acted as catalysts in the whitening process and were solely responsible for activation, where as the lights had no influence. From a laboratory study [6] where approximately 35% HP was used with different light sources (LED/diode laser, a halogen lamp, plasma arc lamp, argon laser) it can be summarized that non-activated whitening did not differ from activated whitening. However, a combination of diode laser treatment with at-home bleaching was reported to provide good results [18]. Another laboratory study reported that bleaching is more effective with a hybrid light-emitting diode (LED) and a low-intensity infrared diode laser than without it [13].

In a laboratory study [8] using six different photoactivation systems on three different 35% hydrogen peroxide whiteners, it was found that only the diode laser, halogen lamp, and LED lamp showed significant color changes. Here it was concluded that the light source is more important than the bleaching agent in the whitening process. Kossatz (2011) [14] reported a larger difference in bleaching with a light-emitting diode [(LED)/laser] than without it (on 35% HP gel), with a shade guide value change of 4.8 vs. 3.8 units. However, tooth sensitivity was higher (53% subjects) for the LED treated group but only 26% for the non-activated group after 24 hours of treatment. Tooth sensitivity was also found to be persistent and higher when the LED activation was used.

In a recent (2011) critical appraisal [19] of power bleaching it was stated that light sources used in tooth whitening do not generate sufficient heat to damage teeth. They concluded that high concentrations of chemicals are responsible for faster whitening and that light sources are therefore superfluous in the whitening process.

The above literature clearly highlights the vast contradiction as to the effect of light activated whitening of teeth. Therefore, the purpose of this study was to determine the whitening effect of a relatively new Light Emitting Diode System (BriteWhite®Inc, Jasper, Alabama, USA).


Twenty one subjects with two sound maxillary teeth (teeth 11 and 21), in good dental and medical health and not on any medical treatment, were selected. Smokers, subjects with fluorosis and tetracycline-stained or previously bleached teeth were excluded from the study. Before the start of the bleaching process teeth were polished with the Brite White polishing tool and paste [20] to remove any stains and plaque, rinsed and blot dried. The color of the teeth was then measured with a spectrophotometer (Model: CM-2600d, Konica Minolta Sensing, Inc., Japan) set on the CIE L*a*b* color space with a 6mm diameter probe. Three measurements were taken (6mm diameter) at the center of the crown of the two different tooth types.

Thereafter the teeth were painted in-chair with the 44% carbamide peroxide gel (which included a photo initiator) using an application brush to achieve maximum tooth contact. A plastic cover was placed over the “U” shaped LED light mouthpiece to prevent contamination. The light was applied for a full cycle of 10 minutes, the gel wiped from the teeth, fresh peroxide gel applied and the light applied again. In this way three full cycles of 10 minutes each were applied.

The in-office session was then completed by having the patient rinse with the Brite White after-rinse solution for 30 seconds. The teeth were then blot dried and the color at the center of each tooth was measured again. Patients were instructed to refrain from eating or drinking foodstuffs rich in color for the first 48 hours after the LED bleaching stage. The patients were requested to note any tooth sensitivity or gingival irritation before treatment, after the LED treatment and after the 14 day at-home treatment period and to rank it in terms of none (1), mild (2), moderate (3) or severe (4).

Customized bleaching trays were provided for each patient and subjects were instructed to apply the 35% carbamide peroxide bleach system daily for 30 minutes for a 14 day period. Patients were instructed to brush gently and not to floss before the bleaching process, according to Brite White instructions. After each at-home application patients were instructed to rinse with water but not to eat or drink anything high in color for at least an hour.

The Wilcox on Signed Rank Sum Test was used to determine the significance of the results.

Full written ethical approval for the project was obtained from the Ethical and Research Committee of the University of the Western Cape.


For the statistical tests we computed ΔL*, Δa*, Δb* and ∆E*ab over time by subtracting the follow-up measurements from baseline measurements [21] and determined the color change by comparing each after bleaching to baseline.

No statistically significant difference in all three components (L*, a* and b*) between teeth 11 and 21 were found. Therefore, the values of teeth 11 and 21 were pooled in the statistical analysis.

Table 1 gives the 25th percentile, median and 75th percentile of the L* values at base-line (before treatment), after the LED treatment, as well as after the 14 day at-home treatment period. It also shows the same for the a* values, and for the b* values over time. Fig. (1) gives the total color change differences (∆E*ab) after the LED treatment as well as after the 14 day at-home treatment.

Fig. (1).

A graph of the 25th percentile (Q1), median (Q2) and 75th percentile (Q3) for the median ΔE*abvalues between base-line (before treatment) and after the LED treatment, as well as between baseline and after the 14 day full treatment period (n=21).

For the L* component (Table 1) a statistically significant difference (p<0.05) in the median values between base-line and just after the LED treatment stage (whiter) was found, but a significant relapse to the original value was also found after the 14 day treatment period (Wilcox on Signed Rank Sum Test). The b* component showed statistically significant differences (p<0.05) between base-line and the LED stage (reduced yellow), between base-line and the 14 day stage (further reduced yellow) as well as between the LED treatment stage and after the 14 day treatment period (least yellow) (Wilcox on Signed Rank Sum Test). For the a* component no statistically significant change was (p>0.05) found throughout (Table 1).

Table 1.

The 25th Percentile, Median (Med) and 75th Percentile Values for L*, a* and b* of 21 Subjects

L* a* b*
25% Med 75% 25% Med 75% 25% Med 75%
Baseline 67.8 69.8 70.3 -0.18 0.04 0.30 6.34 7.54 8.90
After LED 69.3 70.7 71.4 -0.15 0.06 0.31 5.36 6.64 7.93
After 14 days 67.7 69.1 69.7 -0.35 -0.16 0.19 5.44 6.41 8.08

The total color increase (∆E*ab) was 1.8 units after the LED treatment stage with no significant change after the 14 day at-home treatment period (Fig. 1).

Table 2 gives the mean and standard deviation of the tooth sensitivity and gingival sensitivity scores ranked in terms of none (1), mild (2), moderate (3) and severe (4).

Table 2.

Tooth and Gingival Sensitivity Scores of Means with Standard Deviation in Brackets after LED Treatment and after 14 Daystreatment (n=21)

Base-line After LED After 14 days
Teeth S 0.33(0.48) 0.43(0.60) 0.37(0.50)
Gingival S 0.00(0.00) 0.57(0.60) 0.32(0.95)

Both the tooth and gingival sensitivities were found to be very low (below one) as the median values (Table 2) did not even reach a score of one unit (mild). There was a slight insignificant increase in both the sensitivity and gingival scores after the LED activated stage.


Today LED lights are available across the visible, ultraviolet and infrared spectrum of wavelengths. The LED light system investigated in this study [20] is marked as a blue LED light which means the wavelength should be between 450 and 500 nm [22]. It is also reported that LEDs can emit light of an intended color without using any color filters as in traditional lighting methods. This BriteWhite tooth whitening system [20] is claimed to have an activating gel which prevents heat formation, has no sensitivity to teeth, prevents pulp damage, has a blue LED light with a tailored wavelength to activate their custom made gel and can whiten teeth with up to 11 shade tabs within 20 minutes. Furthermore it is FDA cleared while the LED light is also believed to boost the healing of mouth ulcers, cold sores and periodontal diseases.

In this study the color change was measured with a sophisticated spectrophotometer (Model: CM-2600d, Konica Minolta Sensing, Inc., Japan) and not with a shade guide. Using a spectrophotometer has many advantages over shade guides [5, 6, 23-29]. The most important are improved accuracy and quantification of colors by measurement in a three dimensional color space (L*a*b*) [30] [Minolta]. In this space L* indicates lightness/darkness (white/black),a* varies from green (negative side) to red (positive side), while the b* value varies from blue (negative side) to yellow (positive side). On the other hand a shade guide can only provide a total color value, resulting in the loss of important information on the type of color change mentioned above. Furthermore, differences in results can be expected when color evaluation is done with shade guides versus a spectrophotometer as was previously reported [12, 29, 31-36].

The differing results reported with different tooth bleaching systems can be attributed to various factors such as: base-line color of the teeth of the chosen subjects, the type and concentration of the bleaching product, the time period for the in-chair treatment as well as the treatment period and concentration of the products. ThisBriteWhite/LED system can be seen as a mild treatment system for the following reasons: Firstly, the in-chair LED stage treatment uses a relatively low peroxide concentration (44% carbamide peroxide equivalent to ~15% HP) whereas hydrogen peroxide concentrations of approximately 35% are normally used [12, 13, 18]. Secondly, the LED treatment period comprised only 3 ten minute sessions vs. reported [18] 2 twenty minute sessions. Thirdly, the at-home bleaching stage was 14 days, for 30 minutes per day with a low 35% carbamide peroxide (equivalent to ~12% HP) whitener where most at-home bleaching was done for hours over-night [26,27]. The main advantage of such a mild BriteWhite system is that the gingival and sensitivity scores were found to be very low (below mild, Table 1). The large spread of results (Fig. (1) and Table 1) can be attributed to the fact that the base-line color of the teeth was not a selection criteria and the whitening of enamel varies between subjects. Such a large spread of results during whitening has been reported previously [26-28].

Gurgan, et al., 2010 [12] reported a considerably higher total color change than found in this study (∆E*ab= 5.43 vs. our 1.8) also using an LED lamp but with a much higher hydrogen peroxide (38%) concentration and on A3 or darker teeth. This should at least partly explain the higher total color change relative to our study. In agreement with our study their LED lamp also showed a low gingival score (mean 2.9 on a 10 point scale) and low tooth sensitivity (mean 1.1 on a 10 point scale). We only found (Table 2) a small insignificant increase in the tooth sensitivity after the LED treatment, which returned to the base-line value after the 14 day at-home treatment period. However, the gingival score increased from 0 to 0.57 (Table 2) as a result of the LED stage treatment but improved slightly after the 14 day at-home treatment (0.32).

The 14 day at-home bleaching (Fig. 1) could only contribute a further increase of 0.2 units but according to Al-Quar an [18] an improved and more persistent effect could be seen when the bleaching process was longer (3 months).

In a clinical study [12] differences in all 3 the components (ΔL*, Δa* and Δb*) were found for their LED system on 38% HP. The teeth were found to be whiter (ΔL*) with 5.2 units, less red (Δa*) with 0.9 units and less yellow (Δb*) with 1.7 units. These improvements were much higher than found in our study (ΔL*= 1.0; Δa*= 0.02; Δb*=0.9) just after the LED treatment. As found throughout in this study, teeth whitening systems only provided small unit changes in the Δa* values relative to the ΔL* and Δb* values [13, 23-25, 30, 34-39]. In research on stained teeth with 35% HP and LED treatment, where only a ΔL* change was measured, a much higher improvement than in this study (4 units) was reported [7]. In a laboratory study [4] very similar results for ΔL* (4.7) and for a LED light on 35% hydrogen peroxide were reported (ΔL*= 4.7; Δa*= 1.7; Δb*=-3.6 and ∆E*ab =6.4). In another laboratory study [8] also using a LED light and 35% HP, a Vita Shade guide value improvement of about 5.3 units was reported which can be considered relatively high. In this study (Table 1) it can be seen that the b* component was the only component which improved (less yellow) throughout the two application stages, while the L* component initially improved (whiter) and then showed a total relapse to the original value.


This is a low peroxide concentration/LED system. The major tooth color increase was found after the in-office LED/gel treatment stage and only an insignificant further improvement was noted after the additional 14 day at-home treatment period. Only low tooth and gingival sensitivity scores were recorded. A slight increase in both the sensitivity and gingival scores after the LED/gel activated stage could be observed. Overall the total color increase was relatively low.


The BriteWhite® system can be used as an average tooth whitener with low tooth and gingival sensitivity.


This study was financially supported by the DDF fund of the South African Dental Association. Disclaimer: The South African Dental Association does not necessarily support the findings or conclusions made in this article.


The authors confirm that this article content has no conflicts of interest.


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