Toll-Like Receptor 2 Gene Polymorphisms Associated with Aggressive Periodontitis in Japanese

Marika Takahashi 1, 2, Zhiyong Chen 2, 3, 5, Kaoru Watanabe 1, Hiroaki Kobayashi 1, Toshiaki Nakajima 2, 4, Akinori Kimura 2, 4, *, Yuichi Izumi 1
1 Section of Periodontology, Department of Hard Tissue Engineering, Graduate School of Medical and Dental Science
2 Department of Molecular Pathogenesis, Medical Research Institute
3 Department of Vascular and Applied Surgery, Graduate School of Medical and Dental Sciences
4 Laboratory of Genome Diversity, Graduate School of Biomedical Science, Tokyo Medical and Dental University
5 Department of Rheumatology and Immunology, The Affiliated Drum Tower Hospital, Nanjing University Medical School, Nanjing, China

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© Takahashi et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Department of Molecular Pathogenesis, Medical Research Institute, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan; Tel: +81-3-5803-4905; Fax: +81-3-5803-4907, Email:


Background and Objective:

Aggressive periodontitis is a rare and very severe periodontal disease of early onset, which is closely associated with Porphyromonas.gingivalis (P.g.) infection in the Japanese population. TLR2 encodes Toll-like receptor 2, which plays an important role in the protective response to P.g. infection. We investigated a possible association between TLR2 and aggressive periodontitis.

Material and Methods:

Of 2,460 Japanese patients with periodontitis, 38 patients with aggressive periodontitis were enrolled in this study. These 38 aggressive periodontitis patients and 190 Japanese healthy controls were examined for an insertion/deletion (Ins/Del) polymorphism in exon 1, a polymorphism in intron 1 (rs7696323), and a synonymous polymorphism in exon 3 (rs3804100) in TLR2.


We found significant associations of resistance to aggressive periodontitis with the Ins allele (allele frequency in the patients versus controls, 0.540 vs. 0.676, OR=0.56, 95% confidence interval (CI); 0.34-0.92, p=0.022) and the T allele of rs3804100 (0.579 vs. 0.716, OR=0.55, 95% CI; 0.33-0.91, p=0.018), although the C allele of rs7696323 showed no significant association (0.733 vs. 0.829, OR=0.58). A permutation test of Ins/Del-rs7696323-rs3804100 haplotype revealed a significant association between Ins-C-T haplotype (0.252 vs. 0.479, p=0.0003) and resistance to aggressive periodontitis.


The TLR2 polymorphisms were suggested to confer protection against aggressive periodontitis in a Japanese population. The association should be replicated in other cohorts to further identify the responsible TLR polymorphism(s) involved in the pathogenesis of aggressive periodontitis.

Keywords: TLR2, polymorphism, aggressive periodontitis, haplotype.