Structural and Ultra-Structural Features of the First Mandibular Molars of Young Rats Submitted to Pre and Postnatal Protein Deficiencies
L.A Gonçalves, S.C Boldrini*, T.S.O Capote, C.B Binotti, R.A Azeredo, D.T Martini, B Rosenberg, W.G Bautz, E.A Liberti*
Identifiers and Pagination:Year: 2009
First Page: 125
Last Page: 131
Publisher ID: TODENTJ-3-125
Article History:Received Date: 22/10/2008
Revision Received Date: 26/2/2009
Acceptance Date: 8/4/2009
Electronic publication date: 09/6/2009
Collection year: 2009
open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http: //creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
The effects of protein malnutrition, both in utero and prior to weaning, on formation of the first mandibular molars were evaluated by phase-contrast and electron microscopy in rats. The nourished group (GI) received a diet that included 20% casein, while the malnourished group (GII) received 5% casein. The first mandibular molars from GII exhibited low density of cells and odontoblasts, which lacked regular organization compared with molars from GI. In addition, a difference in collagen type was observed between the groups, with a prevalence of Type III collagen fibers detected in the dentin, periodontal ligament, and alveolar bone of GII, and a prevalence of Type I collagen fibers in GI. Finally, examination of surface area in molar sagittal sections indicated 30% less dentin in GII, compared with GI. Our results suggest that structural and ultra-structural features of the dentin-pulp complex and periodontal components of rat molars are affected by protein deficiency.