Evaluation of Remineralizing Effects of CPP-ACP and Nanohydroxyapatite on Erosive Lesions of Enamel in Deciduous Teeth After Exposure to Acetaminophen Syrup: An in vitro Study

1. INTRODUCTION

2. MATERIALS AND METHODS

This is an experimental laboratory investigation on 34 deciduous molar teeth that were previously diagnosed as caries-free (caries detection according to the criteria of WHO), wears, cracks, and hypo calcification in the clinical examination. This sample size was selected based on previous studies [17,20].

The study protocol was approved by the Ethics Committee of Shahed University (Ethics committee reference ID: IR.SHSHED.REC.1397.074). -The surface of the teeth was cleaned from debris by a mechanical method using fluoride-free prophylactic toothpaste with pumice, a low-speed (500-1500 rpm) handpiece, and a brassage-specific brush. [24] Then, a 55 mm square label was applied to the buccal surfaces of the teeth, and all other dental surfaces were covered with clear self-curing acrylic resin. The contact surface of all teeth, regardless of form, size, or group, is identical after hardening. During acrylation, the samples were placed in cold water to prevent changes due to the heat generated by the hardening of the acrylic resin. For proper microhardness measurement, the surface of the samples was polished (abrasive papers of sizes 800, 1000, and 2000; 3M Ind. & Com. Ltda; St Paul, MN, USA) in the presence of water. It allowed the smoothed surface to be analyzed by a Vickers microhardness measuring device. After polishing, the surface of the specimens was dried, and the initial microhardness was measured by the Vickers microhardness tester device (Type M, No g 5025, Shimadzu^® Corporation, Japan). The best point for applying the pressure was determined. Because pressures of 20 and 50 g were used in earlier research to determine the microhardness of teeth, and the pressure of 50 g was shown to be the most appropriate [24], we likewise employed a pressure of 50 g and an 8-second time period. . This pressure was applied at three points, and the microhardness of each one was measured.

After measuring the initial microhardness, all teeth were placed in the Acetaminophen syrup (Corizan, Abidi^® Co, Iran) by pH 5.33 for 1 min [25], three times/ day for seven days. Then, their microhardness was measured for the second time. Then, the teeth were randomly divided into the following groups [26]:

1- Group P.C. (positive control): 2 teeth were placed in fluoride gel (Pascadental, USA) for 10 min.

2- Group NC (negative control): 2 teeth were placed in artificial saliva (Biomaterials Analysis Laboratory of the Faculty of Dentistry, University of Tehran).

3- Group NHA (nanohydroxyapatite): 15 teeth were placed in the 10% nanohydroxyapatite solution (Yazd Pardis Research Company) for 10 min containing 4 g of nano-hydroxyapatite powder and 40 ml of distilled water [17].

4- Group CPP-ACP: 15 teeth were placed in the CPP-ACP (MI Paste, G.C. America) for 10 min [17].

Between phases and prior to microhardness determination, specimens were immersed in artificial saliva. [8]. Then, the microhardness of the teeth was measured for the third time. The microhardness changes within and among the groups were evaluated using the ANOVA test with SPSS software, version 23 (IBM Corp., Armonk, N.Y., USA), and adopting a significant level of 0.05. Multiple comparisons were also performed using the Bonferroni method. Normality of data was tested by Kolmogorov-Smirnova. The p-value was at least 0.2.

3. RESULTS

The mean first microhardness of 15 specimens of the NHA group was 28.303 kgf/mm², with a standard deviation of 51.52. After exposure to the acetaminophen syrup, the value was 252.3 kgf/mm² on average, with a standard deviation of 1.39. The third microhardness of these specimens was 291.24 kgf/mm², with a standard deviation of 82.52. The mean first microhardness of 15 specimens of the CPP-ACP group was 315.99 kgf/mm² with a standard deviation of 54.24, which reached 277.99 kgf/mm² with a standard deviation of 36.53 after exposure to acetaminophen syrup. The third mean microhardness of these specimens was 313.99 kgf/mm² with a standard deviation of 49.38. (Table 1, Fig. 1).

Despite changes in microhardness over the course of the trial, these changes were similar in both groups (NHA and CPP-ACP), and no significant difference in microhardness was seen between the two groups (p-value=0.141). Since changes were significant over time, the value of microhardness was compared in pairs three times in Bonferroni's Paired test (Table 2). The value of microhardness decreased significantly at the second time compared to the first time (p-value=0.001) and it increased (p-value=0.001) after intervention (p-value=0.002). However, after treatment, the microhardness did not show significant changes compared to the initial microhardness (p-value>0.999).

4. DISCUSSION

The effect of nano-hydroxyapatite and CPP-ACP on the microhardness of enamel in deciduous molar teeth following acetaminophen syrup exposure was investigated in this study due to the associated complications of the medication's effects on the enamel and the search for a substance capable of remineralizing the medication.

The study's findings indicate that acetaminophen syrup considerably reduces the enamel's microhardness.

Given that the medical syrups lower the pH, it may explain the reduction of the microhardness after exposure to acetaminophen [27].

Mahmoud and Omar, in their study, showed a positive association between Oral pediatric liquid medications and erosion. These results are in agreement with our study [11]. The results of the study by Babu also showed that in the exposure of enamel in deciduous molar teeth to the four pediatric syrups (amoxicillin, metronidazole, acetaminophen, ibuprofen + acetaminophen), the highest reduction in the microhardness of enamel was observed for the amoxicillin syrup and the lowest for the ibuprofen +acetaminophen [8].

The findings of the study by Lakshay Dhawan are consistent with our study. Despite that, in the study by Lakshay Dhawan, the time of placement of the specimens in the three types of syrup was 1 min, three times/ day for 28 days [25].

This study showed that the microhardness of teeth demineralized with acetaminophen syrup was increased after exposure to nano-hydroxyapatite and CPP-ACP, and nano-hydroxyapatite and CPP-ACP were able to remineralize the demineralized teeth.

Hydroxyapatite is known as a remineralizing agent when applied to the surface of the enamel [28]. Nanohydroxyapatite is a material that is most similar to the tooth structure. It decreases microleakage in dental cavities, is biocompatible, and has antimicrobial properties with remineralization potential [15].Considering the hydrophilic property of the nano-hydroxyapatite crystal, it is a moisturizing agent on the dental surface, which creates a strong layer on the enamel those bonds to the tooth crown [29].

The availability of calcium and phosphorus ions is necessary for the progress of remineralization. The fluorapatite and fluorohydroxyapatite crystals are created in the presence of calcium, phosphorus, and fluoride ions [30]. The microhardness increase in the CPP-ACP group may be due to the amorphous and sedimentable form of calcium and phosphorus ions. On the other hand, saturation with calcium and phosphorus ions converts acidic to alkaline [31]. Also, the results of studies show that ACP-CCP can serve as a calcium source with remineralization capability. The material is designed to stabilize calcium and phosphate ions on the teeth' surface [32-35].

Studies by Rezvani [20] in 2015 and Haghgoo [21] in 2017 showed that the mean microhardness of the enamel of the teeth increases when exposed to the CPP-ACP, which is consistent with our study, but in the study of Rezvani, the permanent teeth were exposed to the coke and whey was compared with CPP-ACP, and in the study of Haghgoo, erosive lesions were created on the permanent molar teeth and TCP was compared with CPP-ACP.

In 2018, Haghgoo showed that nanohydroxyapatite increased the microhardness of the enamel of healthy third molar teeth after exposure to the beer in an in-situ manner, which is consistent with our study, but in this study, the eggshell was compared with nano-hydroxyapatite in an in-situ manner, which is inconsistent with our study [24].

De Carvalho's studies showed that the microhardness of the demineralized enamel increases after the application of nano-hydroxyapatite, in which permanent molars were examined [36]. Additionally, recent research showed hydroxyapatite deposition on polymeric composite resin that could prevent secondary caries on the margins of restorations. [37] Another in vitro and in vivo study demonstrated the high potential of remineralization of the enamel of deciduous teeth when nanostructured microparticles were used in Biomimetic Hydroxyapatite toothpaste [38]. The time of the exposure of specimens to the nano-hydroxyapatite in Mielczarek's study [39] was a 3-week period, in the study of Haghgoo [24], it was 10min, two times/day for ten days, and in another study by the same author, it was 10min [36].

In all studies on this material so far, relatively long exposure times have been applied; however, from the clinical viewpoint, this long period is prohibiting and impossible under clinical conditions. Therefore, in the present study, 10 min was considered for the exposure to remineralizing agents because longer periods would be impossible for patients to use and are not consistent with the clinical criteria [30, 31].

Previous studies [8, 32] with different concentrations of nanohydroxyapatite on the remineralization of initial enamel lesions showed that a concentration of 10% nanohydroxyapatite has a higher remineralization capacity than lower concentrations, but there is no significant difference with higher concentrations.

Some unexplored variables can have a significant influence on the oral environment. The use of probiotics [40] And natural compounds. [41] Can modify Clinical and Microbiological Parameters. These compounds could have an effect also in combination with hydroxyapatite. All these variables should be considered in future clinical trials.

No research has been done on the effect of remineralizing agents on erosive lesions of deciduous teeth caused by medicinal syrups. This study investigated the effects of nano-hydroxyapatite and CPP-ACP on erosive lesions of the enamel of deciduous teeth resulting from the medical syrups. Our study had some limitations, such as finding 34 healthy primary molars.

CONCLUSION

According to the findings of this in vitro study, CPP-ACP and nano-hydroxyapatite can improve enamel's microhardness, which has been reduced as a result of exposure to medicinal syrups.

REFERENCES