CASE REPORT


Guided Bone Regeneration Prior to Implant Therapy in the Esthetic Zone: A Case Report



Benso Sulijaya1, 2, *
iD
, Felix Hartono Koerniadi1
1 Department of Periodontology, Faculty of Dentistry, Universitas Indonesia. Jakarta, Indonesia
2 Research Unit for Oral-Systemic Connection, Division of Oral Science for Health Promotion; Division of Periodontology, Department of Oral Biological Science. Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan


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Creative Commons License
© 2022 Sulijaya and Koerniadi

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Periodontology, Tower B, 2nd Floor. Faculty of Dentistry, Universitas Indonesia. Salemba Raya no. 4. Jakarta Pusat 10430, Indonesia; Tel/Fax; (+6221) 3911502; E-mails: bensosulijaya@gmail.com, bensosulijaya@dent.niigata-u.ac.jp


Abstract

Background:

Implant placement on the anterior maxilla requires a comprehensive concern of adequate alveolar bone and gingival biotype. Insufficient support for hard and soft tissues becomes one of the main causes of implant complications. To avoid this problem, pre-surgical treatment with Guided Bone Regeneration (GBR) is suggested with respect to the alveolar ridge defect.

Objective:

In this study, we reported the predictability of a sandwich technique of GBR prior to implant placement.

Case Presentation:

Case: Thirty-four-year-old male was referred with a chief complaint of missing teeth on area #21 accompanied by labial bone dehiscence due to trauma. Bone thickness measurement displayed was approximately 3/4/5 mm (coronal/middle third/apical third of the alveolar ridge). This case was diagnosed as soft and hard tissue deformity on area #21 prior to implant. Case management: GBR and implant placement were performed in two stages of surgery. GBR was executed by using a combination of Biphasic Calcium Phosphate (BCP) (consisting of 40% Beta-Tri Calcium Phosphate (β-TCP) and 60% Hydroxyapatite (HA)) and Demineralized Freeze-Dried Bone Allografts (DFDBA) bone graft materials. Alveolar ridge re-measurement five months after GBR showed significant bone augmentation, 6/6/6 mm (coronal/middle third/apical third of the alveolar ridge). Furthermore, a 3.5 mm (diameter) x 10 mm (length) bone-level implant was inserted accordingly. The two-year follow-up exhibited gingival stability and no sign of either recession or bone resorption.

Conclusion:

Herein, we demonstrated the effectiveness of BCP and DFDBA bone substitutes to obtain proper conditions for the long-termed stability and predictability of implant.

Keywords: Guided bone regeneration, Implant, Alveolar ridge defect, Case report, BCP, DFDBA.



1. INTRODUCTION

Horizontal bone deficiency in the anterior maxilla is mostly due to periodontitis, trauma, or subsequent to dental extraction [1-3]. Moreover, El-Nahass et al. reported that the mean labial bone thickness at central incisors was less than 1 mm [4]. In cases where ridge preservation is not made right after tooth extraction, the bone dimension is often reduced or even collapsed within a few weeks after tooth extraction [5-7]. Indeed, this condition makes its further management more complex. Of interest to insufficient morphological anatomy to support implant placement, bone augmentation and/or soft tissue grafting therapy are often recommended [1, 3, 8-13].

Various surgical techniques and biomaterials have been developed for the optimization of implant placement in the resorbed alveolar bone. Buser et al. reported that autogenous graft is the best graft material since it exerts potent osseo-conductive and osseo-inductive properties [14]. However, the limited availability of this graft and additional donor site operations are some of the limitations. Interestingly, the combination of bone grafting materials, also named the sandwich technique, has been performed and shown to be beneficial in terms of horizontal or vertical bone gain and implant placement [3, 7, 15, 16]. Moreover, Wang et al. explained that the sandwich technique improved the composition of native bone [17]. Regarding the grafting material, the combination of alloplastic allograft and/or xenograft materials could mimic the natural characteristic of autologous graft [17, 18].

The objective of this case study was to correct bone and soft tissue deformities classified as Seibert class I prior to implant placement. Herein, we demonstrated that the GBR approach using the sandwich technique of BCP and DFDBA achieved an adequate alveolar ridge volume for anterior implant placement. Moreover, we have found that this therapy enforces long-termed stability of dental implants.

2. CASE

A 34 years old male was referred to the Department of Periodontology Clinic, Dental Teaching Hospital, Faculty of Dentistry, Universitas Indonesia, complaining about the anterior missing teeth followed with bone dehiscence. Patient sought to get a fixed prosthetic therapy, which was the dental implant. According to the dental history, tooth loss was caused by an accident that happened 2 years ago. The patient neither had systemic disease nor extra-oral abnormalities.

The intra-oral examination indicated relatively good oral hygiene (Silness and Loe) without any signs of inflammation [19]. Specific to area #21, the length of mesial distal was relatively ideal (8 mm), but not for the labial-palatal dimension. Bone thickness analysis illustrated a horizontal alveolar ridge defect class I according to Seibert classification [20], approximately 3/4/5 mm (coronal/middle third/apical third). Keratinized gingiva and gingival biotypes were 6 mm thick, respectively. Panoramic radiograph revealed no interproximal bone loss (Fig. 1). This case was diagnosed as soft and hard tissue deformity on area #21 prior to implant.

3. CASE MANAGEMENT

After a thorough analysis, we diagnosed this case as a mucogingival deformity and planned to do a GBR to augment the bone prior to implant placement with a good prognosis [21, 22]. The GBR and implant therapy were performed in two stages of surgery. Briefly, local anesthesia of 2% lidocaine (1:50,000 epinephrine) was administrated in the labial and palatal sides. Papilla preservation flap was made in this case. Vertical incisions were made on 2/3 of the mesial angle of adjacent teeth (Fig. 2A). Full thickness flap was elevated. Bone decortication was made using a slow-rotating small diameter bur (Fig. 2B). Then, GBR was conducted by using the sandwich technique of Biphasic Calcium Phosphate (BCP) (40% β-Tri Calcium Phosphate (β-TCP) and 60% Hydroxyapatite (HA)) on the upper part and Demineralized Freeze-Dried Bone Allografts (DFDBA) on the base (Fig. 2C). A long-termed resorbable collagen membrane was applied to cover the bone graft. Nylon 5.0 was used to suture the flap (Fig. 2D). Oral hygiene care instruction was given to the patient and an analgesic was prescribed. A removable denture was placed gently due to aesthetic reasons (Fig. 2E). Patient had consecutive monthly appointments for observation and follow-up. No adverse effect was found.

Five months after the GBR procedure (Fig. 2F), alveolar ridge thickness reassessment exhibited bone volume improvement into 6/6/6 mm (coronal/middle third/apical third of the alveolar ridge). This thickness was quite ideal for anterior implant placement. Then, at the second surgery, the flap was reopened, and a 3.5 mm (diameter) x 10 mm (length) of bone-level implant (Straumman) was inserted accordingly (Fig. 2G-2I). The tension-free flap was closed and sutured using 5.0 nylon (Fig. 2J, K). After the surgery, the patient was instructed to use chlorhexidine gluconate 0.12% mouthwash two times/day for 2 weeks and to take care of personal oral hygiene. Two weeks after the second surgery, no sign of rejection was noticed, after which the stitches were removed. A provisional crown made from the composite resin was screwed to the implant to create a better emergence profile of the gingiva; the final crown was placed later. Twelve months from baseline, the implant was stable without any sign of inflammation or implant tread exposure (Fig. 2L). On the top of that, patient is satisfied with the outcome.

Fig. (1). Intra-oral clinical appearance and panoramic radiograph at baseline.

Fig. (2). A Vertical incisions were made on the 2/3 of mesial angle of adjacent teeth. B Full thickness flap was elevated. Bone decortication was made using a slow-rotating small diameter bur. C Bone graft was applied. D Nylon 5.0 was used to suture the flap. E Removable denture was placed gently. F Five months after GBR procedure. G-K At the second surgery, flap was re-opened, an implant was inserted accordingly, and flap was sutured. L. Twelve months from the baseline.

4. DISCUSSION

In respect to implant placement in the esthetic area with mucogingival deficiencies, several issues must be taken into consideration, such as the height of hard tissue for support, the favorable crown/implant ratio, the amount of keratinized gingiva, and an adequate vascularization for either grafts or dental implant [15, 23, 24]. Herein, the case was diagnosed as Seibert class I ridge defect classification with approximately 3/4/5 mm (coronal/middle third/apical third) bone thickness. According to the guideline for implant therapy, it is important to have 2 mm of the bone surrounding the implant to overcome small bone resorption that might occur during the osseo-integration period [25, 26]. The long junctional epithelium without periodontal fibers is mainly constructed in peri-implant tissue, which is yet another concern [18, 27]. Therefore, the presence of keratinized gingiva as a mechanical barrier function is crucial to give a better peri-implant seal, thereby preventing bacterial invasion [23, 28-32]. This case exhibited an adequate keratinized gingiva with a thick gingival biotype. Therefore, we focused on improving bone volume with a bone graft, which corrects the soft tissue deficiency consequently.

In the present study, we performed GBR and implant placement in two-different operation times. We were concerned about the vascularization matter that affects the integration of either the bone graft to native bone or implant to native bone [6, 14, 16, 33, 34]. Therefore, GBR was established 5 months prior to the implant placement. On the contrary, recent studies demonstrated these procedures simultaneously and observed great precision and accuracy outcomes, thus saving time for the patient and clinician [24, 25]. Unlike our study, Mansour et al. conducted a sandwich osteotomy technique on the cortical bone using a carbide disc, then inserted the implant on the down-fractured segment up to the basal bone [25]. Xenograft materials were filled in the gap between fractured segment, bone, implant, and basal bone, accordingly [35, 36]. In respect to the study done by Mansour et al. [25], the risk of tissue necrotic, which might occur easily due to lack of vascularization and instability of the implant, was our main concern. Taken together, we propose that a less traumatic procedure, adequate blood supply, and healing capacity time through two step surgical phase are desirable.

Although autogenous bone graft remains the gold standard in alveolar reconstructive surgery [14, 16, 20], in this present study, we used a combination of allograft and alloplastic/synthetic grafting material to augment the bone. Briefly, DFDBA, a human allograft, was placed next to the native bone to promote osseo-inductive and conductive functions, while BCP, a mixture of 40% β-TCP and 60% HA, placed on the upper part was served as a scaffold. This idea suggests that its combination brings higher efficacy towards GBR.

Thus, a previous investigation conducted by Miyamoto et al. based on the clinical evidence suggests the goal of the GBR procedure is that peri-implant bone thickness should be more than 1.8-2.0 mm [37]. Moreover, experts recommend even greater peri-implant bone thickness to maintain the stability of the implant [2, 38, 39]. In parallel, the GBR procedure in our study improved the labial bone up to 2-3 mm significantly (Fig. 3). Interestingly, we noticed that this achievement was partly regulated by the membrane characteristics. A long-termed resorbable collagen membrane was used in the present study, in accordance with a previous study done by Sulijaya et al. [3]. It is known that bone regeneration needs at least 4-5 months until it matures, while soft tissue regeneration is relatively faster [13, 16, 40]. In some cases of GBR failure, granulation tissue formation and lack of bone callus formation were often found in this matter [3, 7]. Therefore, the longer resorption time of the membrane allows the bone grafts to regenerate and prevent them from soft tissue proliferation.

Fig. (3). Clinical view and illustration of the bone volume. A Baseline. B Five months after GBR.

This case report has a limitation due to technical issue. The evaluation period is limited up to twelve months due to COVID-19 pandemic situation. The number of patient visits and aerosol generating procedures are reduced in the hospital. However, the evaluation shown here suggests that GBR procedure prior to implant placement is an effective option.

CONCLUSION

In conclusion, GBR with the sandwich technique could be served as a therapy to create an adequate condition for anterior implant placement. Thus, this approach enhances outcome predictability and long-termed implant stability. Longer observations on the stability of dental implants and different bone defect cases will be our consideration for further study.

ETHICS APPROVAL AND CONSENT TO PARTICIPATE

All study protocols were approved by the local ethics committee of the Department of Periodontology, Faculty of Dentistry, Universitas Indonesia as the standard operational procedure (SOP) of the treatment.

HUMAN AND ANIMAL RIGHTS

No animals were used that are the basis of this study.

CONSENT FOR PUBLICATION

Informed consent has been obtained with respect to scientific purposes.

STANDARDS OF REPORTING

CARE guidelines were followed in this study.

AVAILABILITY OF DATA AND MATERIALS

Not applicable.

CONFLICT OF INTEREST

The authors have no conflicts of interest in this study.

FUNDING

No funding has been received for this study.

ACKNOWLEDGEMENTS

The authors thank the Periodontology Unit, Dental Teaching Hospital, Faculty of Dentistry, Universitas Indonesia for the support and facility in performing the case.

REFERENCES

[1] Jensen OT, Kuhlke L, Bedard JF, White D. Alveolar segmental sandwich osteotomy for anterior maxillary vertical augmentation prior to implant placement. J Oral Maxillofac Surg 2006; 64(2): 290-6.
[2] Tonetti MS, Greenwell H, Kornman KS. Staging and grading of periodontitis: Framework and proposal of a new classification and case definition. J Clin Periodontol 2018; 45(January)(Suppl. 20): S149-61.
[3] Sulijaya B, Kuswandani SO, Soeroso Y. Contemporary guided bone regeneration therapy for unaesthetic anterior peri-implantitis case. Dent J 2017; 49(4): 181. [Majalah Kedokt Gigi].
[4] El Nahass H, N Naiem S. Analysis of the dimensions of the labial bone wall in the anterior maxilla: a cone-beam computed tomography study. Clin Oral Implants Res 2015; 26(4): e57-61.
[5] Chen ST, Darby I. The relationship between facial bone wall defects and dimensional alterations of the ridge following flapless tooth extraction in the anterior maxilla. Clin Oral Implants Res 2017; 28(8): 931-7.
[6] Urban I a, Caplanis N, Lozada JL. Simulatneous vertical guided bone regeneration and guided tissue regeneration in the posterior maxilla using recombinant human platelet-derived growth factor: A case report. J Oral Implantol 2009; XXXV(5): 251-6.
[7] Sheikh Z, Sima C, Glogauer M. Bone replacement materials and techniques used for achieving vertical alveolar bone augmentation. Materials (Basel) 2015; 8(6): 2953-93.
[8] Raghoebar GM, Slater JJH, Hartog L, Meijer HJA, Vissink A. Comparison of procedures for immediate reconstruction of large osseous defects resulting from removal of a single tooth to prepare for insertion of an endosseous implant after healing. Int J Oral Maxillofac Implants 2009; 38(7): 736-43.
[9] Kan JYK, Rungcharassaeng K, Sclar A, Lozada JL. Effects of the facial osseous defect morphology on gingival dynamics after immediate tooth replacement and guided bone regeneration: 1-year results. J Oral Maxillofac Surg 2007; 65(7)(Suppl. 1): 13-9.
[10] Lin NH, Gronthos S, Bartold PM. Stem cells and periodontal regeneration. Aust Dent J 2008; 53(2): 108-21.
[11] Slots J. Periodontitis: facts, fallacies and the future. Periodontol 2000 2017; 75(1): 7-23.
[12] Asaad F, Pagni G, Pilipchuk SP, Giannì AB, Giannobile W V, Rasperini G. 3D-printed scaffolds and biomaterials: Review of alveolar bone augmentation and periodontal regeneration applications. Int J Dent 2016; 2016
[13] Reynolds MA, Aichelmann-Reidy ME, Branch-Mays GL. Regeneration of periodontal tissue: bone replacement grafts. Dent Clin North Am 2010; 54(1): 55-71.
[14] Buser D, Hoffmann B, Bernard JP, Lussi A, Mettler D, Schenk RK. Evaluation of filling materials in membrane--protected bone defects. A comparative histomorphometric study in the mandible of miniature pigs. Clin Oral Implants Res 1998; 9(3): 137-50.
[15] Bormann KH, Suarez-Cunqueiro MM, von See C, Kokemüller H, Schumann P, Gellrich NC. Sandwich osteotomy for vertical and transversal augmentation of the posterior mandible. Int J Oral Maxillofac Implants 2010; 39(6): 554-60.
[16] Liu J, Kerns DG. Mechanisms of guided bone regeneration: a review. Open Dent J 2014; 8(Suppl. 1): 56-65.http://benthamopen.com/ABSTRACT/TODENTJ-8-56
[17] Manfrini N, Guerini I, Citterio A, Lucchini G, Longhese MP. Processing of meiotic DNA double strand breaks requires cyclin-dependent kinase and multiple nucleases. J Biol Chem 2010; 285(15): 11628-37.
[18] Caton J, Nyman S, Zander H. Histometric evaluation of periodontal surgery. II. Connective tissue attachment levels after four regenerative procedures. J Clin Periodontol 1980; 7(3): 224-31.
[19] Gupta D, Jain A. Euro Reference and Exchange Rates - Oesterreichische Nationalbank (OeNB). J Int Acad Periodontol 2017; 17(3): 91-8.
[20] Pikos MA. Mandibular block autografts for alveolar ridge augmentation. Atlas Oral Maxillofac Surg Clin North Am 2005; 13(2): 91-107.
[21] Caton JG, Armitage G, Berglundh T, et al. A new classification scheme for periodontal and peri-implant diseases and conditions - Introduction and key changes from the 1999 classification. J Clin Periodontol 2018; 45(March)(Suppl. 20): S1-8.
[22] Hinrichs JE, Kotsakis GA. Classification of diseases and conditions affecting the periodontium.Carranza’s Clinical Periodontology 13th ed. 2019; 55-79.
[23] Monje A, Blasi G. 2018.https://onlinelibrary.wiley.com/doi/abs/10.1002/JPER.18-0471
[24] El Haddad E, Lauritano D, Candotto V, Carinci F. Guided bone regeneration is a reliable technique in implant dentistry: An overview and a case report. Oral Surg 2014; 10(2): 4-7.
[25] Fuglsig JMCES, Thorn JJ, Ingerslev J, Wenzel A, Spin-Neto R. Long term follow-up of titanium implants installed in block-grafted areas: A systematic review. Clin Implant Dent Relat Res 2018; 20(6): 1036-46.http://doi.wiley.com/10.1111/cid.12678
[26] Schwarz F, Derks J, Monje A, Wang HL. Peri-implantitis. J Clin Periodontol 2018; 45(45)(Suppl. 20): S246-66.
[27] Nyman S, Gottlow J, Karring T, Lindhe J. The regenerative potential of the periodontal ligament. An experimental study in the monkey. J Clin Periodontol 1982; 9(3): 257-65.
[28] Yamada M, Takahashi N, Matsuda Y, et al. A bacterial metabolite ameliorates periodontal pathogen-induced gingival epithelial barrier disruption via GPR40 signaling. Sci Rep 2018; 8(1): 9008.http://www.ncbi.nlm.nih.gov/pubmed/29899364%0Ahttp://www.nature.com/articles/s41598-018-27408-y
[29] Groeger SE, Meyle J. Epithelial barrier and oral bacterial infection. Periodontol 2000 2015; 69(1): 46-67.
[30] Takahashi N, Sulijaya B, Yamada-Hara M, Tsuzuno T, Tabeta K, Yamazaki K. Gingival epithelial barrier: regulation by beneficial and harmful microbes. Tissue Barriers 2019; 7(3): e1651158.
[31] Dickinson BC, Moffatt CE, Hagerty D, et al. Interaction of oral bacteria with gingival epithelial cell multilayers. Mol Oral Microbiol 2011; 26(3): 210-20.
[32] Weinberg A, Krisanaprakornkit S, Dale BA. Epithelial antimicrobial peptides: review and significance for oral applications. Crit Rev Oral Biol Med 1998; 9(4): 399-414.
[33] Iwata T, Yamato M, Ishikawa I, Ando T, Okano T. Tissue engineering in periodontal tissue. Anat Rec (Hoboken) 2014; 297(1): 16-25.
[34] Karring T, Nyman S, Gottlow J, Laurell L. Development of the biological concept of guided tissue regeneration--animal and human studies. Periodontol 2000 1993; 1(1): 26-35.
[35] Stavropoulos A, Kostopoulos L, Nyengaard JR, Karring T. Deproteinized bovine bone (Bio-Oss) and bioactive glass (Biogran) arrest bone formation when used as an adjunct to guided tissue regeneration (GTR): an experimental study in the rat. J Clin Periodontol 2003; 30(7): 636-43.
[36] Venezia E, Goldstein M, Boyan BD, Schwartz Z. The use of enamel matrix derivative in the treatment of periodontal defects: a literature review and meta-analysis. Crit Rev Oral Biol Med 2004; 15(6): 382-402.
[37] Yu H, Cui Z, Yu P, et al. PH- and NIR light-responsive micelles with hyperthermia-triggered tumor penetration and cytoplasm drug release to reverse doxorubicin resistance in breast cancer. Adv Funct Mater 2015; 25(17): 2489-500.
[38] Grunder U, Gracis S, Capelli M. Influence of the 3-D bone-to-implant relationship on esthetics. Int J Periodontics Restorative Dent 2005; 25(2): 113-9.
[39] Hadjidakis DJ, Androulakis II. Bone remodeling. Ann N Y Acad Sci 2006; 1092(1092): 385-96.
[40] Cortellini P, Tonetti MS. Clinical concepts for regenerative therapy in intrabony defects. Periodontol 2000 2015; 68(1): 282-307.