Unstimulated Salivary Estrogen in Postmenopausal Women With and Without Oral Dryness: A Prospective Study

Aanchal Gupta1, Shambulingappa Pallagatti2, Deepak Gupta1, *, Amit Aggarwal1, Ravinder Singh1
1 Department of Oral Medicine and Radiology, M.M. College of Dental Sciences and Research, M.M. (Deemed to be University), Mullana, Ambala, India
2 Department of Oral Medicine and Radiology, Bapuji Dental College and Hospital, Davangere, Karnataka, India

Article Metrics

CrossRef Citations:
Total Statistics:

Full-Text HTML Views: 382
Abstract HTML Views: 178
PDF Downloads: 90
ePub Downloads: 54
Total Views/Downloads: 704
Unique Statistics:

Full-Text HTML Views: 230
Abstract HTML Views: 112
PDF Downloads: 68
ePub Downloads: 32
Total Views/Downloads: 442

Creative Commons License
© 2022 Gupta et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Oral Medicine and Radiology, M.M. College of Dental Sciences and Research, M.M. (Deemed to be University), Mullana, Ambala, India; Tel: +91-9896671281;



This study aimed to compare the unstimulated salivary estrogen levels in postmenopausal women with and without oral dryness.


A study was carried out on 70 selected postmenopausal women, out of which 35 were in the case group and 35 were in the control group. A questionnaire related to oral dryness was given to all the patients to evaluate their response to oral dryness. Patients were asked to spit in a plastic container, and their unstimulated saliva samples were obtained and analyzed for estimation of salivary estrogen levels by ELISA technique. After analyzing the results of salivary estrogen levels, the patients in the case group with low levels of estrogen were subjected to HRT under the guidance of a gynecologist. The patients were followed after 3 months of therapy for their response to oral dryness by the questionnaire, and salivary estrogen levels were again reevaluated after the therapy. The statistical analysis was carried out using Statistical Package for Social Sciences (SPSS Inc, Chicago, IL, version 15.0 for windows). The Pearson’s correlation and Student’s unpaired t-test were used for comparisons.


Before hormone replacement therapy, the mean estrogen level of the case group was 1.0031, and the mean estrogen level of the control group was 4.0080 pg/ml. This suggested that the reduced levels of estrogen hormone after menopause were associated with the complaint of oral dryness in these females. The mean salivary estrogen levels in the case group after HRT were more than the levels before HRT.


Estrogen levels were reduced in patients with oral dryness. Further, the patients receiving HRT showed significant improvement in symptoms of oral dryness.

Keywords: Hormone replacement therapy, Oral dryness, Salivary estrogen, Menopause, Postmenopausal women, Estrogen levels.