Evaluation of the Effect of Cold Plasma Treatment on the Microshear Bond Strength of Composite Resin Restorations to Dentin using Different Adhesive Systems and the Effect of Thermocycling

Sara Valizadeh1, Elham Farhadi1, Aida Moradi2, Sedighe S. Hashemikamangar1, *
1 Department of Restorative Dentistry, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
2 Department of Restorative Dentistry, School of Dentistry, Ilam University of Medical Sciences, Ilam, Iran

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© 2021 Valizadeh et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Restorative Dentistry, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran; Tel: 09122403863; E-mail:



Currently, non-thermal plasma is used to modify the enamel and dentin surfaces to improve the bonding surface to dental composite resins. Non-thermal plasma creates a hydrophilic surface, decreases the contact angle, and improves the bonding quality. The present study aimed to evaluate the microshear bond strength (µSBS) of composite resins to dentin using different adhesive systems.

Materials and Methods:

Bovine incisor teeth were randomly assigned to three groups of G-Premio, Clearfil SE Bond, and Adper Single Bond adhesive groups after preparation. Each group was divided into two subgroups in terms of argon plasma surface preparation, and each subgroup was divided into two groups in terms of thermocycling (n=12). The microshear bond strength of the samples was determined using a universal testing machine. Three-way ANOVA was used to analyze the effect of the adhesive, plasma preparation, and thermocycling. Post hoc Tukey tests were used for two-by-two comparisons of µSBS. Statistical significance was set at P <0.05.


The results of the µSBS test showed that the application of plasma resulted in a significant increase in the mean µSBS in the G-Premio group, with no significant increase in the Clearfil SE bond and Adper Single groups. The effect of thermocycling after plasma application was significant only in the Adper Single group.


The application of plasma might increase the bond strength of composite resins to dentin. However, further studies are necessary.

Keywords: Cold atmospheric plasma, Dentin adhesives, Microshear bond strength, Dental composite, Self-etch bonding, Etch and rinse bonding.