Comparison of Biocompatibility of Experimental Tricalcium Phosphate Cement versus Biodentin and Mineral Trioxide Aggregate used for Furcation Perforation Repair (in vivo study)

Mostafa M. El-Bialy1, *, Magdy M. Ali2, Engy M. Kataia1, Reham S. El Nemr3, Reham Hassan4, 5
1 Department of Restorative and Dental Materials, National Research Centre, Cairo, Egypt
2 Department of Endodontics, Faculty of Dentistry, Beni Suef University, Beni Suef, Egypt
3 Department of Pathology, Fayoum University, Faiyum, Egypt
4 Department of Endodontics, Faculty of Dentistry, Minia University, Minya, Egypt
5 Department of Endodontic, Egyptian-Russian University, Badr City, Egypt

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© 2021 El-Bialy et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Restorative and Dental Materials, National Research Centre, Cairo, Egypt; E-mail:



The interaction between the root canal system and the oral cavity caused by iatrogenic perforations significantly affects the treatment outcome and tooth survival.


This study was directed to compare the biocompatibility of an experimental tricalcium phosphate cement versus biodentine and mineral Trioxide aggregate used for furcation perforation repair in dogs.


Perforations were done in 60 teeth (premolars and molars) of six adult dogs. Animals were divided randomly into 3 equal groups of 2 animals each according to the post-operative evaluation period of 1 week, 1 month, and 3-months. Each group was further subdivided into 4 subgroups according to either being repaired with the experimental tricalcium phosphate cement (n=6), or Biodentine (n=6), Mineral Trioxide Aggregate (MTA) (n=6), and positive control(n=2). After evaluation periods, tissue blocks were harvested and histologically examined.


No statistically significant difference was found regarding bone deposition scores and inflammatory reaction in the three groups after 1 week, 1 month, or 3 months. All three groups showed a statistically significant difference between all three time periods. Regardless of the repair material used, inflammation scores at 1-week showed the highest scores, decreasing over time except for the control group.


The experimental material could be considered as a successful treatment option for repairing furcation perforation.

Keywords: Perforation repair, MTA, Biodentine, Experimental tricalcium phosphate cement, Control group, Dogs.