Biological and Immunogenicity Property of IgY Anti S. mutans ComD
E.W. Bachtiar1, *, B.M. Bachtiar1, R.D. Soejoedono2, I.W. Wibawan2, A. Afdhal1
Identifiers and Pagination:Year: 2016
First Page: 308
Last Page: 314
Publisher Id: TODENTJ-10-308
Article History:Received Date: 24/01/2016
Revision Received Date: 01/05/2016
Acceptance Date: 09/05/2016
Electronic publication date: 15/06/2016
Collection year: 2016
open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
This study aims to elucidate the effect of IgY anti ComD on the biological properties of Streptococcus mutans. (S. mutans) ComD is an interspecies quorum-sensing signaling receptor that plays an important role in biofilm formation by S. mutans.
Materials and Methodology:
Egg yolk IgY was produced by the immunization of chickens with a DNA vaccine containing the ComD DNA coding region. We evaluated the effect of the antibody on biofilm formation by S. mutans isolated from subjects with or without dental caries. We also assessed the immunoreactivity of the antibody against all isolates, and analyzed the protein profile of S. mutans by SDS-PAGE.
The ComD antibody was successfully induced in the hens’ eggs. It inhibited biofilm formation by all S. mutans isolates. In addition, the expression of some protein bands was affected after exposure to the antibody.
IgY anti-S. mutans ComD reduces biofilm formation by this bacterium and alters the protein profile of S. mutans.