RESEARCH ARTICLE


The Effect of Root Canal Irrigation with Combination of Sodium Hypo-chlorite and Chlorhexidine Gluconate on the Sealing Ability of Obturation Materials



Hamed Homayouni 1, Nima Moradi Majd 2, Heidar Zohrehei 3, *, Behrad Mosavari 4, Mamak Adel 1, Reyhaneh Dajmar 1, Ali Homayouni 5
1 Department of Endodontics, Dental School, Qazvin University of Medical Sciences, Qazvin, Iran
2 Dental Research Laboratory, Howard University College of Dentistry, Washington DC, USA
3 Dental Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4 Dental Carries Research Center, School of Dentistry, Qazvin University of Medical Sciences, Qazvin, Iran
5 Dental Research Center Dentistry Research Institute, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran


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Creative Commons License
© Homayouni et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Dental Research Center, Shahid Beheshti University of Medical Sciences, Evin, Tehran, Zip code: 1983963113, Iran; Tel: +98 21 22413897; Fax: +98 21 22427753; E-mail: h_zohrehei@yahoo.com


Abstract

Objectives:

The aim of this study was to evaluate the effect of the precipitate that was formed by combining Sodium Hypochlorite (NaOCl) and Chlorhexidine Gluconate (CHX) on the sealing ability of root canal obturation materials.

Materials and Methods:

The fluid filtration method was conducted on a total of 100 roots. Samples were randomly divided into two control (n=5) and three experimental groups (n=30). The samples in group 1 were irrigated with 1.5 mL of 2.5% NaOCl, and then the smear layers of the teeth were removed by 17% EDTA, while the specimens of group 2 were irrigated by 1.5 mL of 2.5% NaOCl and 1.5 mL of 2% CHX; after the smear layer removal, a final flush with 1.5 mL of 2.5% NaOCl was performed. The samples of group 3 were irrigated the same as group 1 but after the smear layer removal canals were irrigated again with 1.5 mL of 2.5% NaOCl and then a final flush with 1.5 mL of 2% CHX was performed. Teeth were obturated with gutta-percha and AH26 sealer and after seven days, microleakage was evaluated by the fluid filtration technique. The results were analyzed by the ANOVA and Tukey's test.

Results:

The samples in group 3 had significantly greater microleakage compared to teeth in group 1, 2 (p<0.05), and the specimens in group 1 showed significantly less amount of microleakage than samples in group 2, 3 (p<0.05).

Conclusion:

The presence of the precipitate that is formed due to interaction between NaOCl and CHX has negative effect on the sealing ability of gutta-percha and AH26 sealer.

Keywords: Chlorhexidine gluconate, Fluid filtration technique, Microleakage, Para-chloroaniline, Sodium hypochlorite.