RESEARCH ARTICLE


Development of A Three-Dimensional Tissue Construct from Dental Human Ectomesenchymal Stem Cells: In Vitro and In Vivo Study



Daniela Guzmán-Uribe1, 2, Keila Neri Alvarado Estrada1, Amaury de Jesús Pozos Guillén3, Silvia Martín Pérez4, Raúl Rosales Ibáñez1, *
1 Grupo de Investigación en Ingeniería Tisular, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, Mexico
2 Posgrado de Estomatología Pediátrica, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, Mexico
3 Laboratorio de Ciencias Básicas, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, Mexico
4 Bioterio General, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, Mexico


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Creative Commons License
© Guzmán-Uribe et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Grupo de Investigación en Ingeniería Tisular, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí., Av. Manuel Nava No. 2, Zona Universitária, Z.P. 78260; Tel: +524448262357; Ext. 114; Fax: +52 444 813 9743; E-mail: raul.rosales@fest.uaslp.mx


Abstract

Application of regenerative medicine technology provides treatment for patients with several clinical problems, like loss of tissue and its function. The investigation of biological tooth replacement, dental tissue engineering and cell culture, scaffolds and growth factors are considered essential. Currently, studies reported on the making of threedimensional tissue constructs focused on the use of animal cells in the early stages of embryogenesis applied to young biomodels. The purpose of this study was the development and characterization of a three-dimensional tissue construct from human dental cells. The construct was detached, cultured and characterized in mesenchymal and epithelial cells of a human tooth germ of a 12 year old patient. The cells were characterized by specific membrane markers (STRO1, CD44), making a biocomplex using Pura Matrix as a scaffold, and it was incubated for four days and transplanted into 30 adult immunosuppressed male Wistar rats. They were evaluated at 6 days, 10 days and 2 months, obtaining histological sections stained with hematoxylin and eosin. Cell cultures were positive for specific membrane markers, showing evident deviations in morphology under phase contrast microscope. Differentiation and organization were noted at 10 days, while the constructs at 2 months showed a clear difference in morphology, organization and cell type. It was possible to obtain a three-dimensional tissue construct from human dental ectomesenchymal cells achieving a degree of tissue organization that corresponds to the presence of cellular stratification and extracellular matrix.

Keywords: Biological tooth replacement, cell culture, cell differentiation, ectomesenchymal dental cells, drop model, three-dimensional tissue constructs, stem cells, human.