In vitro Evaluation of the Stain Removal Efficacy between Two Whitening Dentifrices during Orthodontic Treatment

This in-vitro study aimed to assess and compare the efficacy of a newly developed whitening dentifrice, Dentaklin White (TG), to Colgate Total^® Whitening (CG), utilising a toothbrushing simulator machine.

Twenty enamel specimens were prepared and randomly divided into CG and TG. Orthodontic brackets were attached to the enamel specimens and were then stained with a mixture of coffee, tea, and chlorhexidine. A short 0.019x0.025” stainless steel (SS) archwire was ligated onto the brackets subsequently. All the specimens were subjected to a toothbrushing simulator for specific duration. The CIE L*a*b* colour change (ΔE) was evaluated using digital image analysis at T1 (2 weeks), T2 (4 weeks), and T3 (12 weeks) compared to T0 (baseline). Archwire roughness was analysed using a profilometer at T4 (2 years) compared to T0.

The results indicated that there was a significant change in tooth colour associated with brushing duration for both CG and TG (p<0.05) with no significant difference between the two dentifrices (p>0.05). Both dentifrices notably removed more stains at Site A (p<0.05) and increased wire surface roughness (p<0.05) without a statistically difference between them (p>0.05).

Dentaklin White (TG) demonstrated comparable efficacy to Colgate Total^® Whitening (CG).