The Cell Biocompatibility of a new Polycaprolactone-based Endodontics Sealer Compared with AH Plus Sealer

One of the important materials needed for root treatment is the sealers. They fill the space between the gutta and the canal walls and create a suitable seal to prevent the colonization of oral microorganisms in the peri-apical tissues and inside the canal space.

The aim of this study was to prepare and test the cell biocompatibility of a new polycaprolactone-based endodontics sealer in comparison to AH plus sealer against dental pulp stem cells.

MTT assay was used to evaluate the cytotoxicity.

The results showed that AH plus showed moderate cytotoxicity effects against cells (the number of living cells was 49.45 ± 10.56%). However, for the new sealer, the number of living cells was ≥ 80% (81% ± 4.11). Thus, the sample was non-cytotoxic against tested cells.

More investigations are needed on the new sealer to validate its biocompatibility and usefulness.