Effect of 0.2% Chitosan Associated with Different Final Irrigant Protocols on the Fiber Post Bond Strength to Root Canal Dentin of Bovine Teeth: An In-vitro Study

Maura Cristiane Gonçales Orçati Dorileo1, Ricardo Danil Guiraldo2, Murilo Baena Lopes2, Daniel de Almeida Decurcio3, Orlando Aguirre Guedes4, *, Andreza Maria Fábio Aranha1, Álvaro Henrique Borges1, Alcides Gonini Júnior5
1 Department of Endodontics, Dental School, University of Cuiabá, Cuiabá, Mato Grosso, Brazil
2 Department of Operative Dentistry, Dental School, University of North Paraná, Londrina, Paraná, Brazil
3 Department of Stomatology, Dental School, Federal University of Goiás, Goiânia, Goiás, Brazil
4 Department of Endodontics, Dental School, Evangelical University of Goiás, Anápolis, Goiás, Brazil
5 Department of Operative Dentistry, Dental School, State University of Londrina, Londrina, Paraná, Brazil

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© 2022 Dorileo et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Endodontics, Dental School, Evangelical University of Goiás, Av. Universitária, Km 3,5, Cidade Universitária, 75083-515, Anápolis, Goiás, Brazil; Tel: +55 62 3310-6630; E-mail:



This in-vitro study investigated the effect of 0.2% Chitosan associated with different final irrigant protocols on the bond strength of fiber posts (FP) to root canal dentin.


Fifty bovine incisors roots were prepared using the ProTaper Universal system, irrigated with 2.5% sodium hypochlorite, and divided into one control group (n=10) with no final irrigant protocol and four experimental groups (n=10), which were defined according to the combination of chelating solution (17% EDTA and 0.2% Chitosan) and irrigant activation/delivery method [conventional irrigation (CI), and passive ultrasonic irrigation (PUI)]. Post spaces were prepared to a depth of 12 mm using #1-5 Largo drills, and the FP were cemented using self-adhesive resin cement. Two slices of 2 mm in thickness from each third were obtained and submitted to the micropush-out test. After testing the push-out strength, the slices were analyzed under a stereomicroscope at 40× magnification for bond failure patterns determination. Statistical analysis was performed using three-way ANOVA followed by Tukey’s test (α = 0.05).


The control and 17% EDTA + CI groups exhibited significantly lower bond strength than 0.2% Chitosan + CI, 17% EDTA + PUI, and 0.2% Chitosan + PUI groups in the cervical third (P = 0.00). The cervical third had higher values than the middle and apical thirds in control (P = 0.00), 17% EDTA + PUI (P = 0.00), and 0.2% Chitosan + PUI groups (P = 0.00). Adhesive cement-dentin failure type was predominant in all groups.


The use of 0.2% chitosan did not affect the bond strength of FP to root dentin. Passive ultrasonic activation of chelating solutions resulted in an improvement in bonding strength.

Keywords: Bond strength, Chelating agent, Chitosan, Fiber post, Passive ultrasonic activation, Smear layer.