Evaluation of the Antibacterial Efficacy of Azadirachta Indica, Commiphora Myrrha, Glycyrrhiza Glabra Against Enterococcus Faecalis using Real Time PCR



Suresh Anand1, *, Mathan Rajan2, Nagendrababu Venkateshbabu3, Deivanayagam Kandaswamy2, Yarramreddy Shravya2, Kalaiselvam Rajeswari2
1 Department of Conservative Dentistry and Endodontics, Penang International Dental College, Jalan Bagan Laur,12000, Butterworth, Penang, Malaysia
2 Department of Conservative Dentistry and Endodontics, Faculty of Dental Sciences, Sri Ramachandra University, Chennai, 600 116, India
3 Department of Restorative Dentistry, School of Dentistry, International Medical University, Kuala Lumpur, Malaysia


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© Anand et al. ; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to these authors at the Department of Conservative Dentistry and Endodontics, Penang International Dental College, Jalan Bagan Laur,12000, Butterworth, Penang, Malaysia; Tel : +919597853272; E-mail: andyendo@gmail.com


Abstract

Aim:

To compare the antibacterial efficacy of Azadirachta indica (Neem), Commiphora myrrha (Myrrh), Glycyrrhiza glabra (Liquorice) with 2% Chlorhexidine (CHX) against E. faecalis by using Real Time PCR

Materials and Methods:

A total of fifty teeth specimens (n=50) were inoculated with E. faecalis for 21 days. Specimens were divided into five groups (Group 1: Myrrh, Group 2: Neem, Group 3: Liquorice, Group 4: 2% CHX and Group 5: Saline (negative control)). The intracanal medicaments were packed inside the tooth. After 5 days, the remaining microbial load was determined by using real time PCR

Results:

Threshold cycle (Ct) values of Myrrh extract, Neem extract, Liquorice Extract, 2% CHX and saline were found to be 30.94, 23.85, 21.38, 30.93 and 17.8 respectively

Conclusion:

Myrrh extract showed inhibition of E.faecalis equal to that of 2% CHX followed by Neem, Liquorice and Saline

Keywords: Commiphora myrrha, Neem, Liquorice, 2% CHX, Dentinal tubules, intracanal medicament, real- time polymerase chain reaction.