RESEARCH ARTICLE
Evaluation of the Antibacterial Efficacy of Azadirachta Indica, Commiphora Myrrha, Glycyrrhiza Glabra Against Enterococcus Faecalis using Real Time PCR
Suresh Anand1, *, Mathan Rajan2, Nagendrababu Venkateshbabu3, Deivanayagam Kandaswamy2, Yarramreddy Shravya2, Kalaiselvam Rajeswari2
Article Information
Identifiers and Pagination:
Year: 2016Volume: 10
Issue: Suppl-1, M2
First Page: 160
Last Page: 165
Publisher ID: TODENTJ-10-160
DOI: 10.2174/1874210601610010160
Article History:
Received Date: 30/12/2015Revision Received Date: 10/1/2016
Acceptance Date: 28/1/2016
Electronic publication date: 11/05/2016
Collection year: 2016

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
Abstract
Aim:
To compare the antibacterial efficacy of Azadirachta indica (Neem), Commiphora myrrha (Myrrh), Glycyrrhiza glabra (Liquorice) with 2% Chlorhexidine (CHX) against E. faecalis by using Real Time PCR
Materials and Methods:
A total of fifty teeth specimens (n=50) were inoculated with E. faecalis for 21 days. Specimens were divided into five groups (Group 1: Myrrh, Group 2: Neem, Group 3: Liquorice, Group 4: 2% CHX and Group 5: Saline (negative control)). The intracanal medicaments were packed inside the tooth. After 5 days, the remaining microbial load was determined by using real time PCR
Results:
Threshold cycle (Ct) values of Myrrh extract, Neem extract, Liquorice Extract, 2% CHX and saline were found to be 30.94, 23.85, 21.38, 30.93 and 17.8 respectively
Conclusion:
Myrrh extract showed inhibition of E.faecalis equal to that of 2% CHX followed by Neem, Liquorice and Saline