TODENTJ-25-03-2019_TODENTJ-9-65 1553505630 Bentham Open doi@benthamopen.net Bentham Open The Open Dentistry Journal TODENTJ 1874-2106 1 30 2015 9 1 Torbjørn Breivik Yngvar Gundersen Robert Murison Jonathan D Turner Claude P Muller Per Gjermo Kristian Opstad Background and Objective: Early life adverse events may influence susceptibility/resistance to chronic inflammatory diseases later in life by permanently dysregulating brain-controlled immune-regulatory systems. We have investigated the impact of infant-mother separation during early postnatal life on the severity of experimental periodontitis, as well as systemic stress and immune responses, in adulthood. Material and Methods: Pups of periodontitis resistant Lewis rats were separated from their mothers for 3 h daily during postnatal days 2-14 (termed maternal deprivation; MD), separated for 15 min daily during the same time period (termed handling; HD), or left undisturbed. As adults, their behaviour was tested in a novel stressful situation, and ligature-induced periodontitis applied for 21 days. Two h before sacrifice all rats were exposed to a gram-negative bacterial lipopolysaccharide (LPS) challenge to induce a robust immune and stress response. Results: Compared to undisturbed controls, MD rats developed significantly more periodontal bone loss as adults, whereas HD rats showed a tendency to less disease. MD and HD rats exhibited depression-like behaviour in a novel open field test, while MD rats showed higher glucocorticoid receptor (Gr) expression in the hippocampus, and HD rats had altered methylation of genes involved in the expression of hippocampal Gr. LPS provoked a significantly lower increase in circulating levels of the cytokine TGF-1β in MD and HD rats, but there were no significant differences in levels of the stress hormone corticosterone. Conclusion: Stressful environmental exposures in very early life may alter immune responses in a manner that influences susceptibility/resistance to periodontitis. 1 30 2015 65 78 TODENTJ-9-65 1 10.2174/crossmark_policy opendentistryjournal.com benthamopen.com true Peer Reviewed Single blind Checked with iThenticate 2014-10-28 2014-11-24 2014-12-11 2015-1-30 http://creativecommons.org/licenses/by-nc/3.0/ 10.2174/1874210601509010065 1553505630 https://opendentistryjournal.com/VOLUME/9/PAGE/65/ https://opendentistryjournal.com/contents/volumes/V9/TODENTJ-9-65/TODENTJ-9-65.pdf https://opendentistryjournal.com/contents/volumes/V9/TODENTJ-9-65/TODENTJ-9-65.pdf https://opendentistryjournal.com/contents/volumes/V9/TODENTJ-9-65/TODENTJ-9-65.pdf https://opendentistryjournal.com/contents/volumes/V9/TODENTJ-9-65/TODENTJ-9-65.pdf https://opendentistryjournal.com/contents/volumes/V9/TODENTJ-9-65/TODENTJ-9-65.pdf https://opendentistryjournal.com/contents/volumes/V9/TODENTJ-9-65/TODENTJ-9-65.pdf https://opendentistryjournal.com/contents/volumes/V9/TODENTJ-9-65/TODENTJ-9-65.pdf https://opendentistryjournal.com/contents/volumes/V9/TODENTJ-9-65/TODENTJ-9-65.xml Periodontol Socransky SS 38 135 2000 Socransky SS, Haffajee AD. 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