Bactericidal Efficacy of Cold Plasma at Different Depths of Infected Root Canals In Vitro

TODENTJ-25-03-2019_TODENTJ-9-486 1553505944 Bentham Open doi@benthamopen.net Bentham Open The Open Dentistry Journal TODENTJ 1874-2106 12 31 2015 9 1 Bactericidal Efficacy of Cold Plasma at Different Depths of Infected Root Canals In Vitro Sascha R. Herbst Moritz Hertel Husam Ballout Philipp Pierdzioch Klaus-Dieter Weltmann Henrik C. Wirtz Shady Abu-Sirhan Eckehard Kostka Sebastian Paris Saskia Preissner Objectives: Cold plasma (CP) has been shown to be effective even against multiresistant microorganisms. As previous investigations on the effect of CP in root canals showed promising results, the aim of the present study was to analyze the bactericidal efficacy of CP in different depths of infected dentin. Methods: 32 standardized root canals of human mandibular premolars were infected with Enterococcus faecalis and incubated for one week. Specimens were randomly selected for one of four disinfection methods: control (5mL NaCl), 5mL chlorhexidine (CHX), CP alone (CP), and a combination of 5mL CHX and cold plasma (CHX+CP). CHX was ultrasonically activated for 30s, while cold plasma was used for 60s in the root canals. Dentin samples at depths of 300, 500 and 800 µm were obtained and diluted serially. Colony forming units (CFUs) were counted on agar plates after 24h of incubation. Results: The highest overall logarithmic reduction factors (RF) were obtained from CHX+CP (log RF 3.56 p<0.01; Mann-Whitney U test), followed by CP (log RF 3.27 p<0.01) and CHX alone (log RF 2.65 p<0.01) related to the control. All disinfection methods showed significantly lower CFU counts compared to the control group in 300 µm and 800 µm (both p<0.01, Kruskal-Wallis test). Discussion: The adjuvant use of CP might be beneficial in highly infected root canals to improved disinfection. However, the disinfection effect against Enterococcus faecalis of CP is comparable to ultrasonically activated CHX. 12 31 2015 486 491 TODENTJ-9-486 1 10.2174/crossmark_policy opendentistryjournal.com benthamopen.com true Peer Reviewed Single blind Checked with iThenticate 2015-6-17 2015-11-3 2015-11-6 2015-12-31 https://creativecommons.org/licenses/by/4.0/legalcode 10.2174/1874210601509010486 1553505944 https://opendentistryjournal.com/VOLUME/9/PAGE/486/ https://opendentistryjournal.com/contents/volumes/V9/TODENTJ-9-486/TODENTJ-9-486.pdf https://opendentistryjournal.com/contents/volumes/V9/TODENTJ-9-486/TODENTJ-9-486.pdf https://opendentistryjournal.com/contents/volumes/V9/TODENTJ-9-486/TODENTJ-9-486.pdf https://opendentistryjournal.com/contents/volumes/V9/TODENTJ-9-486/TODENTJ-9-486.pdf https://opendentistryjournal.com/contents/volumes/V9/TODENTJ-9-486/TODENTJ-9-486.pdf https://opendentistryjournal.com/contents/volumes/V9/TODENTJ-9-486/TODENTJ-9-486.pdf https://opendentistryjournal.com/contents/volumes/V9/TODENTJ-9-486/TODENTJ-9-486.pdf https://opendentistryjournal.com/contents/volumes/V9/TODENTJ-9-486/TODENTJ-9-486.xml J. 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