TODENTJ-25-03-2019_TODENTJ-9-396 1553505910 Bentham Open doi@benthamopen.net Bentham Open The Open Dentistry Journal TODENTJ 1874-2106 12 11 2015 9 1 Markus Tobias Winfried Zaruba Tilla Filli Dan-Krister Rechenberg Thomas Thurnheer Thomas Attin Patrick Roger Schmidlin Purpose: To measure the release of an antibiotic mixture of ciprofloxacin, cerfuroxim and metronidazole (TreVitaMix, TVM) through human dentine and to assess the growth inhibition of Fusobacterium nucleatum. Material and Methods: Twenty-four extracted human incisors were scaled and endodontically treated. Root canals were either filled with antibiotic tri-mixture (TVM) or with the carrier material alone (propylene glycol, PG) and were coronally and apically sealed with a flowable composite. Transradicular medicament release was spectrophotometrically measured at 277 nm in simulated body fluid for up to 21 days. In a second part, an agar diffusion assay (F. nucleatum) with representative TVM concentrations as determined in the first part was performed to study the growth inhibition. Samples were anaerobical incubated for 48 h and inhibition zones were measured. Results: TVM was spectrophotometrically detectable in the immersion solution and released in decreasing concentrations up to 21 days (222.5 ± 65.2 mg/ml at day 1 and 35.1 ± 15.6 mg/ml at day 21). In addition, inhibition zones were shown in the agar diffusion assay at representative TVM concentrations. The carrier material showed no antibacterial effect. Conlusion: TVM showed the potential to penetrate through dentine and to inhibit bacterial growth. Therefore, it might have the potential to disinfect the outer root surface in perio-endo lesions, but further research is needed to confirm these observations. 12 11 2015 396 401 TODENTJ-9-396 1 10.2174/crossmark_policy opendentistryjournal.com benthamopen.com true Peer Reviewed Single blind Checked with iThenticate 2015-4-2 2015-8-24 2015-9-29 2015-12-11 https://creativecommons.org/licenses/by/4.0/legalcode 10.2174/1874210601509010396 1553505910 https://opendentistryjournal.com/VOLUME/9/PAGE/396/ https://opendentistryjournal.com/contents/volumes/V9/TODENTJ-9-396/TODENTJ-9-396.pdf https://opendentistryjournal.com/contents/volumes/V9/TODENTJ-9-396/TODENTJ-9-396.pdf https://opendentistryjournal.com/contents/volumes/V9/TODENTJ-9-396/TODENTJ-9-396.pdf https://opendentistryjournal.com/contents/volumes/V9/TODENTJ-9-396/TODENTJ-9-396.pdf https://opendentistryjournal.com/contents/volumes/V9/TODENTJ-9-396/TODENTJ-9-396.pdf https://opendentistryjournal.com/contents/volumes/V9/TODENTJ-9-396/TODENTJ-9-396.pdf https://opendentistryjournal.com/contents/volumes/V9/TODENTJ-9-396/TODENTJ-9-396.pdf https://opendentistryjournal.com/contents/volumes/V9/TODENTJ-9-396/TODENTJ-9-396.xml J. 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