TODENTJ-25-03-2019_TODENTJ-8-184 1553505566 Bentham Open doi@benthamopen.net Bentham Open The Open Dentistry Journal TODENTJ 1874-2106 10 22 2014 8 1 Hamed Homayouni Nima Moradi Majd Heidar Zohrehei Behrad Mosavari Mamak Adel Reyhaneh Dajmar Ali Homayouni Objectives: The aim of this study was to evaluate the effect of the precipitate that was formed by combining Sodium Hypochlorite (NaOCl) and Chlorhexidine Gluconate (CHX) on the sealing ability of root canal obturation materials. Materials and Methods: The fluid filtration method was conducted on a total of 100 roots. Samples were randomly divided into two control (n=5) and three experimental groups (n=30). The samples in group 1 were irrigated with 1.5 mL of 2.5% NaOCl, and then the smear layers of the teeth were removed by 17% EDTA, while the specimens of group 2 were irrigated by 1.5 mL of 2.5% NaOCl and 1.5 mL of 2% CHX; after the smear layer removal, a final flush with 1.5 mL of 2.5% NaOCl was performed. The samples of group 3 were irrigated the same as group 1 but after the smear layer removal canals were irrigated again with 1.5 mL of 2.5% NaOCl and then a final flush with 1.5 mL of 2% CHX was performed. Teeth were obturated with gutta-percha and AH26 sealer and after seven days, microleakage was evaluated by the fluid filtration technique. The results were analyzed by the ANOVA and Tukey's test. Results: The samples in group 3 had significantly greater microleakage compared to teeth in group 1, 2 (p<0.05), and the specimens in group 1 showed significantly less amount of microleakage than samples in group 2, 3 (p<0.05). Conclusion: The presence of the precipitate that is formed due to interaction between NaOCl and CHX has negative effect on the sealing ability of gutta-percha and AH26 sealer. 10 22 2014 184 187 TODENTJ-8-184 1 10.2174/crossmark_policy opendentistryjournal.com benthamopen.com true Peer Reviewed Single blind Checked with iThenticate 2014-7-16 2014-8-11 2014-8-14 2014-10-22 http://creativecommons.org/licenses/by-nc/3.0/ 10.2174/1874210601408010184 1553505566 https://opendentistryjournal.com/VOLUME/8/PAGE/184/ https://opendentistryjournal.com/contents/volumes/V8/TODENTJ-8-184/TODENTJ-8-184.pdf https://opendentistryjournal.com/contents/volumes/V8/TODENTJ-8-184/TODENTJ-8-184.pdf https://opendentistryjournal.com/contents/volumes/V8/TODENTJ-8-184/TODENTJ-8-184.pdf https://opendentistryjournal.com/contents/volumes/V8/TODENTJ-8-184/TODENTJ-8-184.pdf https://opendentistryjournal.com/contents/volumes/V8/TODENTJ-8-184/TODENTJ-8-184.pdf https://opendentistryjournal.com/contents/volumes/V8/TODENTJ-8-184/TODENTJ-8-184.pdf https://opendentistryjournal.com/contents/volumes/V8/TODENTJ-8-184/TODENTJ-8-184.pdf https://opendentistryjournal.com/contents/volumes/V8/TODENTJ-8-184/TODENTJ-8-184.xml Oral Surg Oral Med Oral Patho Kakehashi S 20 340 1965 Kakehashi S, Stanley HR, Fitzgerald RJ. 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