TODENTJ-25-03-2019_TODENTJ-7-7 1553505439 Bentham Open doi@benthamopen.net Bentham Open The Open Dentistry Journal TODENTJ 1874-2106 2 22 2013 7 1 Aristidis Arhakis Vasilis Karagiannis Sotirios Kalfas The secretion of salivary alpha-amylase (sAA) is more associated with psychoneuroendocrinological response to stress than with the flow rate and age. The aim of this cross sectional study is to build an explanatory model based on patterns of relationship between age 20-39 in resting and stimulated saliva under no stressful condition in healthy volunteers. Both resting and stimulated saliva were collected from 40 subjects. The sAA values were log-transformed, the normality assumption was verified with the Shapiro-Wilk test and the reliability of the measurements was estimated by the Pearsons’ r correlation coefficient. The estimated model was based on the theory of the Linear Mixed Models. Significant mean changes were observed in flow rate and sAA activity between resting and stimulated saliva. The final model consists of two components, the first revealed a positive correlation between age and sAA while the second one revealed a negative correlation between the interaction of age × flow rate in its condition (resting or stimulated saliva), with sAA. Both flow rate and age influence sAA activity. 2 22 2013 7 15 TODENTJ-7-7 1 10.2174/crossmark_policy opendentistryjournal.com benthamopen.com true Peer Reviewed Single blind Checked with iThenticate 2012-9-25 2012-11-28 2012-12-18 2013-2-22 http://creativecommons.org/licenses/by-nc/3.0/ 10.2174/1874210601307010007 1553505439 https://opendentistryjournal.com/VOLUME/7/PAGE/7/ https://opendentistryjournal.com/contents/volumes/V7/TODENTJ-7-7/TODENTJ-7-7.pdf https://opendentistryjournal.com/contents/volumes/V7/TODENTJ-7-7/TODENTJ-7-7.pdf https://opendentistryjournal.com/contents/volumes/V7/TODENTJ-7-7/TODENTJ-7-7.pdf https://opendentistryjournal.com/contents/volumes/V7/TODENTJ-7-7/TODENTJ-7-7.pdf https://opendentistryjournal.com/contents/volumes/V7/TODENTJ-7-7/TODENTJ-7-7.pdf https://opendentistryjournal.com/contents/volumes/V7/TODENTJ-7-7/TODENTJ-7-7.pdf https://opendentistryjournal.com/contents/volumes/V7/TODENTJ-7-7/TODENTJ-7-7.pdf https://opendentistryjournal.com/contents/volumes/V7/TODENTJ-7-7/TODENTJ-7-7.xml Ann N Y Acad Sci Baum BJ 694 17 1993 Baum BJ. Principles of saliva secretion Ann N Y Acad Sci 1993; 694 : 17-23. Crit Rev Oral Biol Med Castle D 9 4 1998 Castle D, Castle A. Intracellular transport and secretion of salivary proteins Crit Rev Oral Biol Med 1998; 9 : 4-22. Crit Rev Clin Lab Sci Zakowski JJ 21 283 1985 Zakowski JJ, Bruns DE. Biochemistry of human alpha amylase isoenzymes Crit Rev Clin Lab Sci 1985; 21 : 283-322. Psychosom Med Bosch JA 65 245 2003 Bosch JA, De Geus EJ, Veerman EC, Hoogstraten J, Nieuw Amerongen AV. Innate secretory immunity in response to laboratory stressors that evoke distinct patterns of cardiac autonomic activity Psychosom Med 2003; 65 : 245-58. Horm Metab Res Gilman S 11 454 1979 Gilman S, Thornton R, Miller D, Biersner R. Effects of exercise stress on parotid gland secretion Horm Metab Res 1979; 11 : 454. Scand J Clin Lab Invest Nexø E 48 269 1988 Nexø E, Hansen MR, Konradsen L. Human salivary epidermal growth factor, haptocorrin and amylase before and after prolonged exercise Scand J Clin Lab Invest 1988; 48 : 269-73. Eur J Oral Sci Steerenberg PA 105 305 1997 Steerenberg PA, Van Asperen IA, van Nieuw Amerongen A, et al. Salivary levels of immunoglobulin A in triathletes Eur J Oral Sci 1997; 105 : 305-9. J Clin Endocrinol Metab Chatterton RT 82 2503 1997 Chatterton RT Jr, Vogelsong KM, Lu YC, Hudgens GA. Hormonal responses to psychological stress in men preparing for skydiving J Clin Endocrinol Metab 1997; 82 : 2503-9. J Sports Sci Walsh NP 17 129 1999 Walsh NP, Blannin AK, Clark AM, et al. The effects of high-intensity intermittent exercise on saliva IgA, total protein and alpha-amylase J Sports Sci 1999; 17 : 129-34. Psychoneuroendocrinology Van Stegeren A 31 137 2006 Van Stegeren A, Rohleder N, Everaerd W, Wolf OT. Salivary alpha amylase as marker for adrenergic activity during stress: effect of betablockade Psychoneuroendocrinology 2006; 31 : 137-41. Psychoneuroendocrinology Kivlighan KT 31 703 2006 Kivlighan KT, Granger DA. Salivary alpha-amylase response to competition: relation to gender, previous experience, and attitudes Psychoneuroendocrinology 2006; 31 : 703-14. Ann N Y Acad Sci Rohleder N 1032 258 2004 Rohleder N, Nater UM, Wolf JM, Ehlert U, Kirschbaum C. Psychosocial stress-induced activation of salivary alpha-amylase: an indicator of sympathetic activity? Ann N Y Acad Sci 2004; 1032 : 258-63. Psychoneuroendocrinology Gordis EB 31 976 2006 Gordis EB, Granger DA, Susman EJ, Trickett PK. Asymmetry between salivary cortisol and alpha-amylase reactivity to stress: relation to aggressive behavior in adolescents Psychoneuroendocrinology 2006; 31 : 976-87. Int J Psychophysiol Nater UM 55 333 2005 Nater UM, Rohleder N, Gaab J, et al. Human salivary alpha-amylase reactivity in a psychosocial stress paradigm Int J Psychophysiol 2005; 55 : 333-42. Psychoneuroendocrinology Nater UM 31 49 2006 Nater UM, La Marca R, Florin L, et al. Stress-induced changes in human salivary alpha-amylase activity-associations with adrenergic activity Psychoneuroendocrinology 2006; 31 : 49-58. Clin Physiol Chatterton RTJR 16 433 1996 Chatterton RTJR, Vogelsong KM, Lu YC, Ellman AB, Hudgens GA. Salivary alpha-amylase as a measure of endogenous adrenergic activity Clin Physiol 1996; 16 : 433-8. Clin Oral Investig Bergdahl M 6 145 2002 Bergdahl M, Bergdahl J. Perceived taste disturbance in adults: prevalence and association with oral and psychological factors and medication Clin Oral Investig 2002; 6 : 145-9. Arch Oral Biol Dezan CC 47 423 2002 Dezan CC, Nicolau J, Souza DN, Walter LR. Flow rate, amylase activity, and protein and sialic acid concentrations of saliva from children aged 18, 30 and 42 months attending a baby clinic Arch Oral Biol 2002; 47 : 423-7. Oral Surg Oral Med Oral Pathol Oral Radiol Endod Enberg N 92 292 2001 Enberg N, Alho H, Loimaranta V, Lenander-Lumikari M. Saliva flow rate, amylase activity, and protein and electrolyte concentrations in saliva after acute alcohol consumption Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2001; 92 : 292-8. J Sports Sci Allgrove JE 26 653 2008 Allgrove JE, Gomes E, Hough J, Gleeson M. Effects of exercise intensity on salivary antimicrobial proteins and markers of stress in active men J Sports Sci 2008; 26 : 653-1. Indian J Dent Res Chitra S 19 213 2008 Chitra S, Shyamala Devi CS. Effects of radiation and alphatocopherol on saliva flow rate, amylase activity, total protein and electrolyte levels in oral cavity cancer Indian J Dent Res 2008; 19 : 213-8. Psychophysiology Rohleder N 43 645 2006 Rohleder N, Wolf JM, Maldonado EF, Kirschbaum C. The psychosocial stress -induced increase in salivary alpha-amylase is independent of saliva flow rate Psychophysiology 2006; 43 : 645-52. Physiol Behav Mackie DA 47 593 1990 Mackie DA, Pangborn RM. Mastication and its influence on human salivary flow and alpha-amylase secretion Physiol Behav 1990; 47 : 593-. Physiol Behav Beltzer EK 101 289 2010 Beltzer EK, Fortunato CK, Guaderrama MM, et al. Salivary flow and alpha-amylase: collection technique, duration, and oral fluid type Physiol Behav 2010; 101 : 289-96. Psychoneuroendocrinology Nater UM 32 392 2007 Nater UM, Rohleder N, Schlotz W, Ehlert U, Kirschbaum C. Determinants of the diurnal course of salivary alpha-amylase Psychoneuroendocrinology 2007; 32 : 392-401. J Endocrinol Messenger B 177 407 2003 Messenger B, Clifford MN, Morgan LM. Glucose-dependent insulinotropic polypeptide and insulin-like immunoreactivity in saliva following sham-fed and swallowed meals J Endocrinol 2003; 177 : 407-12. Hum Exp Toxicol Zappacosta B 21 7 2002 Zappacosta B, Persichilli S, Mordente A, et al. Inhibition of salivary enzymes by cigarette smoke and the protective role of glutathione Hum Exp Toxicol 2002; 21 : 7-11. Int J Dent Hyg Brand HS 4 160 2006 Brand HS, Bruins ML, Veerman EC, Nieuw Amerongen AV. Secretion rate and amylase concentration of whole saliva after consumption of beer Int J Dent Hyg 2006; 4 : 160-. Ann NY Acad Sci ?avazesh M 694 72 1993 ?avazesh M. Methods for collecting saliva Ann NY Acad Sci 1993; 694 : 72-. Ann NY Acad Sci Granger DA 1098 122 2007 Granger DA, Kivlighan KT, El-Sheikh M, Gordis EB, Stroud LR. Salivary alpha-amylase in biobehavioral research: recent developments and applications Ann NY Acad Sci 2007; 1098 : 122-44. Clin Chem Winn-Deen ES 34 2005 1988 Winn-Deen ES, David H, Sigler G, Chavez R. Development of a direct assay for alpha-amylase Clin Chem 1988; 34 : 2005-8. Psychoneuroendocrinology Rohleder N 34 469 2009 Rohleder N, Nater UM. Determinants of salivary alpha-amylase in humans and methodological considerations Psychoneuroendocrinology 2009; 34 : 469-85. Psychoneuroendocrinology Pruessner JC 28 916 2003 Pruessner JC, Kirschbaum C, Meinlschmid G, Hellhammer DH. Two formulas for computation of the area under the curve represent measures of total hormone concentration versus time-dependent change Psychoneuroendocrinology 2003; 28 : 916-31. West B, Welch K, Galecki A. Linear mixed models a practical guide using statistical software. Boca Raton: Chapman & Hall/CRC 2007. Qual Life Res 11 193 2002 Scientific Advisory Committee of the Medical Outcomes Trust Source. Assessing health status and quality-of-life instruments: attributes and review criteria Qual Life Res 2002; 11 : 193-205. Auton Neurosci Proctor GB 133 3 2007 Proctor GB, Carpenter GH. Regulation of salivary gland function by autonomic nerves Auton Neurosci 2007; 133 : 3-18. J Dent Res Garrett JR 66 387 1987 Garrett JR. The proper role of nerves in salivary secretion: a review J Dent Res 1987; 66 : 387-97. Psychosom Med Bosch JA 58 374 1996 Bosch JA, Brand HS, Ligtenberg TJ, et al. Psychological stress as a determinant of protein levels and salivary-induced aggregation of Streptococcus gordonii in human whole saliva Psychosom Med 1996; 58 : 374-82. Int J Psychophysiol Skosnik PD 36 59 2000 Skosnik PD, Chatterton RT JR, Swisher T, Park S. Modulation of attentional inhibition by norepinephrine and cortisol after psychological stress Int J Psychophysiol 2000; 36 : 59-68. Arch Oral Biol Takai N 49 963 2004 Takai N, Yamaguchi M, Aragaki T, et al. Effect of psychological stress on the salivary cortisol and amylase levels in healthy young adults Arch Oral Biol 2004; 49 : 963-8. Psychoneuroendocrinology Bosch JA 36 449 2011 Bosch JA, Veerman EC, De Geus EJ, Proctor GB. α-Amylase as a reliable and convenient measure of sympathetic activity: don't start salivating just yet! Psychoneuroendocrinology 2011; 36 : 449-53. Clin Endocrinol Gallina S 75 747 2011 Gallina S, Di Mauro M, D'amico MA, et al. Salivary chromogranin A, but not α-amylase, correlates with cardiovascular parameters during high-intensity exercise Clin Endocrinol 2011; 75 : 747-52. Physiol Behav Froehlich DA 41 209 1987 Froehlich DA, Pangborn RM, Whitaker JR. The effect of oral stimulation on human parotid salivary flow rate and alpha-amylase secretion Physiol Behav 1987; 41 : 209-17. J Physiol Dawes C 183 360 1966 Dawes C. The composition f human saliva secreted in response to a gustatory stimulus and to pilocaprine J Physiol 1966; 183 : 360-8. Crit Rev Oral Biol Med Schenkels LC 6 161 1995 Schenkels LC, Veerman EC, Nieuw Amerongen AV. Biochemical composition of human saliva in relation to other mucosal fluids Crit Rev Oral Biol Med 1995; 6 : 161-75. J Prosthet Dent Humphrey SP 85 162 2001 Humphrey SP, Williamson RT. A review of saliva: normal composition, flow, and function J Prosthet Dent 2001; 85 : 162-9. Eur J Oral Sci Veerman EC 104 346 1996 Veerman EC, Van Den Keybus PA, Vissink A, Nieuw Amerongen AV. Human glandular salivas: their separate collection and analysis Eur J Oral Sci 1996; 104 : 346-52. Arch Oral Biol Bellavia SL 24 117 1979 Bellavia SL, Moreno J, Sanz E, Picas EI, Blanco A. alpha-Amylase activity of human neonate and adult saliva Arch Oral Biol 1979; 24 : 117-21. Pediatr Res Hodge C 17 998 1983 Hodge C, Lebenthal E, Lee PC, Topper W. Amylase in the saliva and in the gastric aspirates of premature infants: its potential role in glucose polymer hydrolysis Pediatr Res 1983; 17 : 998-1001. Am J Clin Nutr Sevenhuysen GP 39 584 1984 Sevenhuysen GP, Holodinsky C, Dawes C. Development of salivary alpha-amylase in infants from birth to 5 months Am J Clin Nutr 1984; 39 : 584-98. Psychoneuroendocrinology Davis EP 34 795 2009 Davis EP, Granger DA. Developmental differences in infant salivary alpha-amylase and cortisol responses to stress Psychoneuroendocrinology 2009; 34 : 795-804. Dev Psychobiol Yim IS 52 598 2010 Yim IS, Granger DA, Quas JA. Children's and adults' salivary alpha-amylase responses to a laboratory stressor and to verbal recall of the stressor Dev Psychobiol 2010; 52 : 598-602. Biochem Med Metab Biol Ben-Aryeh H 36 260 1986 Ben-Aryeh H, Shalev A, Szargel R, et al. The salivary flow rate and composition of whole and parotid resting and stimulated saliva in young and old healthy subjects Biochem Med Metab Biol 1986; 36 : 260-5. Arch Oral Biol Aguirre A 32 297 1987 Aguirre A, Levine MJ, Cohen RE, Tabak LA. Immunochemical quantitation of alpha-amylase and secretory IgA in parotid saliva from people of various ages Arch Oral Biol 1987; 32 : 297-301. J Dent Res Ghezzi EM 82 844 2003 Ghezzi EM, Ship JA. Aging and secretory reserve capacity of major salivary glands J Dent Res 2003; 82 : 844-. Arch Oral Biol Nagler RM 50 7 2005 Nagler RM, Hershkovich O. Relationships between age, drugs, oral sensorial complaints and salivary profile Arch Oral Biol 2005; 50 : 7-16.