TODENTJ-25-03-2019_TODENTJ-6-235 1553505423 Bentham Open doi@benthamopen.net Bentham Open The Open Dentistry Journal TODENTJ 1874-2106 12 28 2012 6 1 Tarek El-Bialy Adel Alhadlaq Brian Lam The objective of this study was to investigate whether low intensity pulsed ultrasound (LIPUS) has anabolic effects on human periodontal ligament (PDL) cells. The PDL cells were plated in 48-well plates and cultured at 37°C in an atmosphere of 5% CO2 in air, in a humidified incubator until confluent. The cells were divided into three groups including control, 5 min and 10 min ultrasound application. The LIPUS was applied using a 2.5 transducer that produces an incident intensity of 30 mW/cm2 of the transducer's surface area. The results from the quantitative polymerase chain reaction (PCR) indicates that expression levels of alkaline phosphatase (ALP),cyclin D1 (CYC), nucleostemin (NCT) were increased after four weeks of 10 minutes of daily ultrasound treatment. The increased ALP/DNA by LIPUS shows a time dependent pattern with the highest activity occurring after four weeks of treatment. These results demonstrate that LIPUS has an anabolic effect on PDL cells and suggest that LIPUS may enhance the pluripotent characteristics of PDL cells as indicated by the up-regulation of NCT, a stem marker. These results also may explain the potential role of LIPUS in periodontal tissue regeneration. 12 28 2012 235 239 TODENTJ-6-235 1 10.2174/crossmark_policy opendentistryjournal.com benthamopen.com true Peer Reviewed Single blind Checked with iThenticate 2012-7-5 2012-8-10 2012-9-27 2012-12-28 http://creativecommons.org/licenses/by-nc/3.0/ 10.2174/1874210601206010235 1553505423 https://opendentistryjournal.com/VOLUME/6/PAGE/235/ https://opendentistryjournal.com/contents/volumes/V6/TODENTJ-6-235/TODENTJ-6-235.pdf https://opendentistryjournal.com/contents/volumes/V6/TODENTJ-6-235/TODENTJ-6-235.pdf https://opendentistryjournal.com/contents/volumes/V6/TODENTJ-6-235/TODENTJ-6-235.pdf https://opendentistryjournal.com/contents/volumes/V6/TODENTJ-6-235/TODENTJ-6-235.pdf https://opendentistryjournal.com/contents/volumes/V6/TODENTJ-6-235/TODENTJ-6-235.pdf https://opendentistryjournal.com/contents/volumes/V6/TODENTJ-6-235/TODENTJ-6-235.pdf https://opendentistryjournal.com/contents/volumes/V6/TODENTJ-6-235/TODENTJ-6-235.pdf https://opendentistryjournal.com/contents/volumes/V6/TODENTJ-6-235/TODENTJ-6-235.xml Periodontol Nanci A 40 11 2006 Nanci A, Bosshardt DD. 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