TODENTJ-25-03-2019_TODENTJ-6-226 1553505419 Bentham Open doi@benthamopen.net Bentham Open The Open Dentistry Journal TODENTJ 1874-2106 12 28 2012 6 1 Daniela Guzmán-Uribe Keila Neri Alvarado Estrada Amaury de Jesús Pozos Guillén Silvia Martín Pérez Raúl Rosales Ibáñez Application of regenerative medicine technology provides treatment for patients with several clinical problems, like loss of tissue and its function. The investigation of biological tooth replacement, dental tissue engineering and cell culture, scaffolds and growth factors are considered essential. Currently, studies reported on the making of threedimensional tissue constructs focused on the use of animal cells in the early stages of embryogenesis applied to young biomodels. The purpose of this study was the development and characterization of a three-dimensional tissue construct from human dental cells. The construct was detached, cultured and characterized in mesenchymal and epithelial cells of a human tooth germ of a 12 year old patient. The cells were characterized by specific membrane markers (STRO1, CD44), making a biocomplex using Pura Matrix as a scaffold, and it was incubated for four days and transplanted into 30 adult immunosuppressed male Wistar rats. They were evaluated at 6 days, 10 days and 2 months, obtaining histological sections stained with hematoxylin and eosin. Cell cultures were positive for specific membrane markers, showing evident deviations in morphology under phase contrast microscope. Differentiation and organization were noted at 10 days, while the constructs at 2 months showed a clear difference in morphology, organization and cell type. It was possible to obtain a three-dimensional tissue construct from human dental ectomesenchymal cells achieving a degree of tissue organization that corresponds to the presence of cellular stratification and extracellular matrix. 12 28 2012 226 234 TODENTJ-6-226 1 10.2174/crossmark_policy opendentistryjournal.com benthamopen.com true Peer Reviewed Single blind Checked with iThenticate 2012-7-5 2012-8-10 2012-9-27 2012-12-28 http://creativecommons.org/licenses/by-nc/3.0/ 10.2174/1874210601206010226 1553505419 https://opendentistryjournal.com/VOLUME/6/PAGE/226/ https://opendentistryjournal.com/contents/volumes/V6/TODENTJ-6-226/TODENTJ-6-226.pdf https://opendentistryjournal.com/contents/volumes/V6/TODENTJ-6-226/TODENTJ-6-226.pdf https://opendentistryjournal.com/contents/volumes/V6/TODENTJ-6-226/TODENTJ-6-226.pdf https://opendentistryjournal.com/contents/volumes/V6/TODENTJ-6-226/TODENTJ-6-226.pdf https://opendentistryjournal.com/contents/volumes/V6/TODENTJ-6-226/TODENTJ-6-226.pdf https://opendentistryjournal.com/contents/volumes/V6/TODENTJ-6-226/TODENTJ-6-226.pdf https://opendentistryjournal.com/contents/volumes/V6/TODENTJ-6-226/TODENTJ-6-226.pdf https://opendentistryjournal.com/contents/volumes/V6/TODENTJ-6-226/TODENTJ-6-226.xml Curr Opin Biotechnol Atala A 20 575 2009 Atala A. 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