TODENTJ-25-03-2019_TODENTJ-5-52 1553505242 Bentham Open doi@benthamopen.net Bentham Open The Open Dentistry Journal TODENTJ 1874-2106 3 29 2011 5 1 Seyed Mostafa Mousavinasab Mohammad Atai Bagher Alavi Objectives: To compare the microleakage among experimental adhesives containing nanoclay fillers after the storages of 24 hours and 6 months. Materials and Methods: Class V cavities were prepared on extracted human molars with the occlusal margins located in enamel and the cervical margins in cementum. Phosphoric acid was applied to the enamel and dentin margins.Subsequently, the cavities were treated using four groups of experimental adhesive systems and restored with a resin composite. Adper Single Bond® was used as control group. After 24- hour and 6- month storages, the samples were subjected to thermocycling shocks and then immersed in silver nitrate as well as developer solution and finally evaluated for leakage. The data were analyzed using SPSS software. Results: Based on Kruskal –Wallis test, significant differences were found between groups regarding microleakage. The Mann- Whitney test showed that Leakage was significantly lower in Adper Single Bond® compared to the other groups in dentinal margins after 24 hours and 6 months and in enamel margins after 6 months. The Wilcoxon Signed Ranks test showed that the enamel leakage in experimental adhesives was significantly lower than dentinal leakage after 24 hours as well as enamel leakage in Adper Single Bond and adhesive with 0.5% PMAA-g-nanoclay was significantly lower than dentinal margins after storage period of 6 months. Conclusion: All the experimental adhesives were effective in reducing enamel leakage after 24 hours, but were not effective in reducing dentinal leakage after 24 hours as well as in enamel and dentinal leakage after a 6-month storage. No improvement was observed in the microleakage in dentin in both short (24 hrs) and long times (6 months). The high microleakage in the adhesives is probably attributed to the high concentration of HEMA in the recipe of the bonding agent. 3 29 2011 52 57 TODENTJ-5-52 1 10.2174/crossmark_policy opendentistryjournal.com benthamopen.com true Peer Reviewed Single blind Checked with iThenticate 2010-8-19 2010-11-16 2010-11-24 2011-3-29 http://creativecommons.org/licenses/by-nc/3.0/ 10.2174/1874210601105010052 1553505242 https://opendentistryjournal.com/VOLUME/5/PAGE/52/ https://opendentistryjournal.com/contents/volumes/V5/TODENTJ-5-52/TODENTJ-5-52.pdf https://opendentistryjournal.com/contents/volumes/V5/TODENTJ-5-52/TODENTJ-5-52.pdf https://opendentistryjournal.com/contents/volumes/V5/TODENTJ-5-52/TODENTJ-5-52.pdf https://opendentistryjournal.com/contents/volumes/V5/TODENTJ-5-52/TODENTJ-5-52.pdf https://opendentistryjournal.com/contents/volumes/V5/TODENTJ-5-52/TODENTJ-5-52.pdf https://opendentistryjournal.com/contents/volumes/V5/TODENTJ-5-52/TODENTJ-5-52.pdf https://opendentistryjournal.com/contents/volumes/V5/TODENTJ-5-52/TODENTJ-5-52.pdf https://opendentistryjournal.com/contents/volumes/V5/TODENTJ-5-52/TODENTJ-5-52.xml Quintessence Int Ben Amar A 36 177 2005 Ben Amar A, shapinko E. A microleakage study of single bottle adhesives applied to enamel and cementum and aged by both occlusal loading and thermocycling Quintessence Int 2005; 36 : 177-82. Summit JB, Robbins JW, Hilton TJ, Schwartz RS, Santos JD Jr. Fundamentals of operative dentistry. 3rd. Hanover Park: Quintessence 2006; pp. 183-260. Compend Contin Educ Dent Boghosian A 17 750 1996 Boghosian A. Clinical evaluation of a filled adhesive system in class 5 restorations Compend Contin Educ Dent 1996; 17 : 750-2. J Contemp Dent Pract Harada TS 7 34 2006 Harada TS, Pazinatto FB, Wang L, Atta MT. Effect of number coats of simplified adhesive systems on microleakage of dentin bordered composite restorative J Contemp Dent Pract 2006; 7 : 34-41. Oper Dent Pongprueksa P 32 466 2007 Pongprueksa P, Kuphasuk W, Senawongse P. Effect of elastic cavity wall and occlusal loading on microleakage and dentin Bond strength Oper Dent 2007; 32 : 466-75. Am J Dent Carrilho MR 17 104 2004 Carrilho MR, Carvalho RM, Tay FR, Pashley DH. Effect of storage media on mechanical properties of adhesive systems Am J Dent 2004; 17 : 104-8. J Dent Res De Munk J 84 118 2005 De Munk J, Van Landuyt K, Pumans M. A critical review of the durability of adhesion to tooth tissue: methods and results J Dent Res 2005; 84 : 118-32. Dent Mater Armstrong SR 17 268 2001 Armstrong SR, Keller JC, Boyer DB. The influence of water storage and C- factor on the dentin- resin composite microtensile bond strength and debond pathway utilizing a filled and unfilled adhesive resin Dent Mater 2001; 17 : 268-76. J Dent Res Van Meerbeek B 73 1690 1994 Van Meerbeek B, Peumance M, Verschueren M. Clinical status of ten dentin adhesive systems J Dent Res 1994; 73 : 1690-702. Quintessence Int Van Meerbeek B 27 775 1996 Van Meerbeek B, Peumance M, Gladys S, Braem M, lambrechts P, Vanherle G. Three-year clinical effectiveness of four total-etch dentinal adhesive systems in cervical lesions Quintessence Int 1996; 27 : 775-84. J Dent Opdam NJ 32 379 2004 Opdam NJ, Loomancs BA, Roeters FJ, Bronkhorst EM. Five year clinical performance of posterior resin composite restorations place by dental student J Dent 2004; 32 : 379-83. Am J Dent Nunes MF 14 340 2001 Nunes MF, Swift EJJR, Perdiago J. Effect of adhesive composition on microtensile bond strength to human dentin Am J Dent 2001; 14 : 340-. J Dent Montes MA 29 435 2001 Montes MA, de Goes MF, da Cunha MR, Soares AB. A morphological and tensile bond strength evaluation of unfilled adhesive with low viscosity composites and a filled adhesive in one and two coats J Dent 2001; 29 : 435-1. Dent Mater Miazaki M 11 234 1995 Miazaki M, Ando S, Hinoura K, Onose H, Moore BK. Influence of filler addition to bonding agent to shear bond strength to bovin dentin Dent Mater 1995; 11 : 234-8. J Biomed Mater Res B Appl Biomater Kim JS 72 284 2005 Kim JS, Cho BH, Lee IB, et al. Effect of the hydrophilic nanofiller loading on the mechanical properties and the microtensile bond strength of an ethanol-based one-bottle dentin adhesive J Biomed Mater Res B Appl Biomater 2005; 72 : 284-91. Dent Mater Lee JH 22 515 2006 Lee JH, Um CM, Lee IB. Rheological properties of resin composites according to variation in monomer and filler composition Dent Mater 2006; 22 : 515-26. Dent Mater Ellakwa A 23 1229 2007 Ellakwa A, Cho N, Lee IB. The effect of resin matrix composition on the polymerization shrinkage and rheological properties of experimental dental composite Dent Mater 2007; 23 : 1229-35. Acta Biomater Chivrac F 4 1707 2008 Chivrac F, Gueguen O, Pollet E, Ahzi S, Makradi A, Averous L. Micromechanical modeling and characterization of the effective properties in starch-based nano-biocomposites Acta Biomater 2008; 4 : 1707-4. Dent Mater Atai M 25 339 2009 Atai M, Solhi L, Nodehi A, Mirabedini SM, Kasraei S, Akbari K. PMMA-grafted nanoclay as novel filler for dental adhesives Dent Mater 2009; 25 : 339-47. J Dent Conde MC 37 331 2009 Conde MC, Zanchi CH, Rodrigues-Junior SA, Carreño NL, Ogliari FA, Piva E. Nanofiller loading level: Influence on selected properties of an adhesive resin J Dent 2009; 37 : 331-5. J Dent Dowling AH 34 802 2006 Dowling AH, Stamboulis A, Fleming GJ. The influence of montmorillonite clay reinforcement on the performance of a glass ionomer restorative J Dent 2006; 34 : 802-10. Pesqui odontol Bras Sadek FT 17 261 2003 Sadek FT, Moura SK, Ballester RY, Muench A, Cardoso PE. The effecte of long term storage on the microleakage on composite resin restoration qualitative and quantitative evaluation Pesqui odontol Bras 2003; 17 : 261-6. Am J Dent Gwinnet AJ 8 109 1995 Gwinnet AJ, YU S. Effect of long-term water storage on dentin bonding Am J Dent 1995; 8 : 109-1. Quintessence Int Crim GA 22 387 1991 Crim GA. Microleakage of three dentinal bonding systems: a 6-month evaluation Quintessence Int 1991; 22 : 387-9. Am J Dent Crim GA 6 192 1993 Crim GA. Effect of aging on microleakage of restorative system Am J Dent 1993; 6 : 192-4. J Dent Res Hashimoto M 79 1385 2000 Hashimoto M, Ohno H, Kaga M, Endo K, Sano H, Oguchi H. In vivo degradation of resin-dentin bond in humans over 1 to 3 years J Dent Res 2000; 79 : 1385-91. J Prosthet Dent Wahab FK 90 168 2003 Wahab FK, Shaini FJ, Morgano SM. The effect of thermocycling on microleakage of several commercially available composite Class V restorations in vitro J Prosthet Dent 2003; 90 : 168-74. Dent Mater Van Landuyt KL 24 1412 2008 Van Landuyt KL, Snauwaert J, Peumans M, De Munck J, Lambrechts P, Van Meerbeek B. The role of HEMA in one-step self-etch adhesives Dent Mater 2008; 24 : 1412-9. J Dent Torkabadi S 36 80 2008 Torkabadi S, Nakajima M, Ikeda M, Foxton RM, Tagami J. Bonding durability of HEMA-free and HEMA-containing one-step adhesives to dentine surrounded by bonded enamel J Dent 2008; 36 : 80-6. Biomaterials Atai M 26 5015 2005 Atai M, Watts DC, Atai Z. Shrinkage strain-rates of dental resin-monomer and composite systems Biomaterials 2005; 26 : 5015-20. J Dent Mine A 36 767 2008 Mine A, De Munk J, Van Landuyt KL, Poitevin A, Kuboki T, Yoshida T. Bonding effectiveness and interfacial characterization of a HEMA/TEGDMA-free three-step etch & rinse adhesive J Dent 2008; 36 : 767-3. J Contemp Dent Pract Ateyah NZ 5 63 2004 Ateyah NZ, Elhejazi AA. Shear Bond strength and microleakage of four types of dentin adhesive materials J Contemp Dent Pract 2004; 5 : 63-73. J Am Dent Assoc Costa Pfeifer CS 137 197 2006 Costa Pfeifer CS, Braga RR, Cardoso PE. Influence of cavity dimensions, insertion technique and adhesive system on microleakage of class V restorations J Am Dent Assoc 2006; 137 : 197-202.