In vitro Evaluation of the Cytotoxicity of Different Root Canal Filling Materials

TODENTJ-25-03-2019_TODENTJ-5-29 1553505234 Bentham Open doi@benthamopen.net Bentham Open The Open Dentistry Journal TODENTJ 1874-2106 3 16 2011 5 1 In vitro Evaluation of the Cytotoxicity of Different Root Canal Filling Materials Gianluca Gambarini Luca Testarelli Dina Al-Sudani Gianluca Plotino Nicola M Grande Alessandro Lupi Bruno Giardina Giuseppina Nocca Massimo De Luca Objective: Aim of the present study was to evaluate the cytotoxicity of Real Seal 1 compared to other commercially available endodontic filling materials: Real Seal (SybronEndo, Orange, CA, USA) and Thermafil (Tulsa Dental, Tulsa, OK, USA). Material and Methods: Periodontal ligament cells from healthy patients were cultured. The eluate of Real Seal 1TM (RS1), Real Seal (RS) and Thermafil (TF) samples was used for the cells viability tests, both diluted (50%) or undiluted (100%). Incubation of the specimens was performed in culture medium for 24 h, 48 h and 72 h at 37 °C under sterile conditions. The cellular mortality was evaluated by MTT test. Results were statistically analysed and the statistical significance was set at p< 0.05. Results: None of the studied materials showed toxic effects during the period of observation (0 -72 h) when compared to the control group. Only RS induced a very modest increase in cell mortality (about 3% at both concentrations used, during the first 24 hours), when increasing the incubation time, however, only the lower concentration continued to show modest toxicity. Conclusions: Results of the present study showed that all tested materials did not exhibit cytotoxic effects when compared to the control group. 3 16 2011 29 32 TODENTJ-5-29 1 10.2174/crossmark_policy opendentistryjournal.com benthamopen.com true Peer Reviewed Single blind Checked with iThenticate 2010-8-8 2010-11-11 2010-11-29 2011-3-16 http://creativecommons.org/licenses/by-nc/3.0/ 10.2174/1874210601105010029 1553505234 https://opendentistryjournal.com/VOLUME/5/PAGE/29/ https://opendentistryjournal.com/contents/volumes/V5/TODENTJ-5-29/TODENTJ-5-29.pdf https://opendentistryjournal.com/contents/volumes/V5/TODENTJ-5-29/TODENTJ-5-29.pdf https://opendentistryjournal.com/contents/volumes/V5/TODENTJ-5-29/TODENTJ-5-29.pdf https://opendentistryjournal.com/contents/volumes/V5/TODENTJ-5-29/TODENTJ-5-29.pdf https://opendentistryjournal.com/contents/volumes/V5/TODENTJ-5-29/TODENTJ-5-29.pdf https://opendentistryjournal.com/contents/volumes/V5/TODENTJ-5-29/TODENTJ-5-29.pdf https://opendentistryjournal.com/contents/volumes/V5/TODENTJ-5-29/TODENTJ-5-29.pdf https://opendentistryjournal.com/contents/volumes/V5/TODENTJ-5-29/TODENTJ-5-29.xml Clin Oral Invest Geurtsen W 1 5 1997 Geurtsen W, Leyhausen G. 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