TODENTJ-25-03-2019_TODENTJ-5-110 1553505260 Bentham Open doi@benthamopen.net Bentham Open The Open Dentistry Journal TODENTJ 1874-2106 7 7 2011 5 1 Åsa Leonhardt Anette Carlén Lisbeth Bengtsson Gunnar Dahlén The aim was to compare the detection frequency of periodontopathogens by using the Pado Test 4.5 and checkerboard DNA-DNA hybridization technique in chronic periodontitis patients. Thirty patients with chronic periodontitis were tested cross-sectionally with DNA/RNA oligogenomic probe method (IAI Pado Test 4.5) and DNA/DNA whole genomic probe (checkerboard) method. Samples were taken by two paper points at the deepest site in each of the four quadrants and pooled into one sample for each of the two methods. The samples were sent to the two laboratories (IAI, Zuchwil, Switzerland, and Oral Microbiology Laboratory, University of Gothenburg, Sweden) and were analyzed in a routine setting for the presence and amount of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola. While Pado Test 4.5 detected the four periodontal pathogens in 11 (36.7%) of the patients, the checkerboard method showed presence in all patients (100%) using the lower score (Score 1 corresponding to 104 bacterial cells) and 16 (53.3%) using a higher treshold (score 3 corresponding to between >105 and 106 cells). The results of the present study showed low agreement for a positive microbiological outcome using the two diagnostic methods. It was also concluded that microbiological analysis in practice should include a larger number of bacterial species to better serve as markers for a diseased associated flora in chronic periodontitis cases. 7 7 2011 110 115 TODENTJ-5-110 1 10.2174/crossmark_policy opendentistryjournal.com benthamopen.com true Peer Reviewed Single blind Checked with iThenticate 2011-1-5 2011-3-19 2011-4-8 2011-7-7 http://creativecommons.org/licenses/by-nc/3.0/ 10.2174/1874210601105010110 1553505260 https://opendentistryjournal.com/VOLUME/5/PAGE/110/ https://opendentistryjournal.com/contents/volumes/V5/TODENTJ-5-110/TODENTJ-5-110.pdf https://opendentistryjournal.com/contents/volumes/V5/TODENTJ-5-110/TODENTJ-5-110.pdf https://opendentistryjournal.com/contents/volumes/V5/TODENTJ-5-110/TODENTJ-5-110.pdf https://opendentistryjournal.com/contents/volumes/V5/TODENTJ-5-110/TODENTJ-5-110.pdf https://opendentistryjournal.com/contents/volumes/V5/TODENTJ-5-110/TODENTJ-5-110.pdf https://opendentistryjournal.com/contents/volumes/V5/TODENTJ-5-110/TODENTJ-5-110.pdf https://opendentistryjournal.com/contents/volumes/V5/TODENTJ-5-110/TODENTJ-5-110.pdf https://opendentistryjournal.com/contents/volumes/V5/TODENTJ-5-110/TODENTJ-5-110.xml J Clin Periodontol Brochut PF 32 695 2005 Brochut PF, Marin I, Baehni P, Mombelli A. 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