TODENTJ-25-03-2019_TODENTJ-4-140 1553505180 Bentham Open doi@benthamopen.net Bentham Open The Open Dentistry Journal TODENTJ 1874-2106 7 20 2010 4 1 Germano Orrù Susanna Del Nero Enrica Tuveri Maria Laura Ciusa Francesca Pilia Matteo Erriu Ginevra Orrù Manuele Liciardi Vincenzo Piras Gloria Denotti A dental unit water line (DUWL) equipped with a device designed to automatically and continually flush a bacteriostatic solution of hydrogen peroxide (WHE) and a discontinuous disinfecting system (BIOSTER) was evaluated. In the first instance a preliminary sensitivity test on a large number of microorganisms (bacteria and fungi) was tried with a H2O2 range from 100 to 800 ppm. The bacteria frequently reported in DUWL (including Pseudomonas spp, Streptococcus spp., Staphylococcus spp., E. coli) and some periodontal pathogens showed a minimum inhibitory concentration from 100 to 300 H2O2 ppm (also including M. marinum and C. albicans). However, H2O2 did not show any inhibitory effects against: A. actinomycetemcomitans, C. glabrata C. parapsilos, F. nucleatum, M. micros. In a second step, the DUWL was experimentally infected with S. faecalis, E. coli, P. aeruginosa, S. aureus. After disinfection steps with 3% H2O2, the inhibitory effect on planktonic forms and on sessile biofilm was measured. In a third step, the count of 16S rRNA gene copies by real time PCR at different points of the DUWL described an accrue of bacterial slime in “hot spot” regions characterized by irregular/slow water flux (valves, elbows). However these results suggest that hydrogen peroxide is not only able to inhibit bursts of planktonic bacteria inside the DUWL, but that it could also be effective against sessile biofilm containing heterotrophic microorganisms derived from domestic water line contamination. In addition some oral pathogens could be contaminating and surviving in DUWL. 7 20 2010 140 146 TODENTJ-4-140 1 10.2174/crossmark_policy opendentistryjournal.com benthamopen.com true Peer Reviewed Single blind Checked with iThenticate 2009-11-13 2010-2-15 2010-2-25 2010-7-20 http://creativecommons.org/licenses/by-nc/3.0/ 10.2174/1874210601004010140 1553505180 https://opendentistryjournal.com/VOLUME/4/PAGE/140/ https://opendentistryjournal.com/contents/volumes/V4/TODENTJ-4-140/TODENTJ-4-140.pdf https://opendentistryjournal.com/contents/volumes/V4/TODENTJ-4-140/TODENTJ-4-140.pdf https://opendentistryjournal.com/contents/volumes/V4/TODENTJ-4-140/TODENTJ-4-140.pdf https://opendentistryjournal.com/contents/volumes/V4/TODENTJ-4-140/TODENTJ-4-140.pdf https://opendentistryjournal.com/contents/volumes/V4/TODENTJ-4-140/TODENTJ-4-140.pdf https://opendentistryjournal.com/contents/volumes/V4/TODENTJ-4-140/TODENTJ-4-140.pdf https://opendentistryjournal.com/contents/volumes/V4/TODENTJ-4-140/TODENTJ-4-140.pdf https://opendentistryjournal.com/contents/volumes/V4/TODENTJ-4-140/TODENTJ-4-140.xml Compend Contin Educ Dent Molinari JA 20 358 1999 Molinari JA. 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