Bacterial 16S rRNA/rDNA Profiling in the Liquid Phase of Human Saliva

TODENTJ-25-03-2019_TODENTJ-3-80 1553505053 Bentham Open doi@benthamopen.net Bentham Open The Open Dentistry Journal TODENTJ 1874-2106 4 28 2009 3 1 Bacterial 16S rRNA/rDNA Profiling in the Liquid Phase of Human Saliva F Gu Y Li C Zhou D.T.W Wong C.M Ho F Qi W Shi Human saliva can be separated by centrifugation into cell pellet and cell-free supernatant, which are called cellular phase and liquid phase in this study. While it is well documented that the cellular phase of saliva contains hundreds of oral bacteria species, little is known whether the liquid phase of saliva contains any information related to oral microbiota. In this study, we analyzed the bacterial nucleic acid contents of the liquid phase of saliva. Using primers universal to most eubacterial 16S rDNA, we detected large amounts of bacterial 16S rRNA and rDNA in the cell-free phase of saliva. Random sequencing analysis of forty PCR amplicons from the cell-free phase of saliva led to 15 operational taxonomic unit (OTU) groups. Furthermore, using denaturing gradient gel electrophoresis (DGGE), we compared 16S rRNA/rDNA profiles derived from liquid phases and cellular phases of saliva samples, and found positive correlations (Pearson Correlation=0.822, P<0.001) between these sample groups. These findings indicate that the liquid phase of saliva contains numerous bacterial 16S rRNA/rDNA molecules that have correlations with bacteria existing in the cellular phase. 4 28 2009 80 84 TODENTJ-3-80 1 10.2174/crossmark_policy opendentistryjournal.com benthamopen.com true Peer Reviewed Single blind Checked with iThenticate 2008-8-20 2008-12-23 2009-3-26 2009-4-28 http://creativecommons.org/licenses/by-nc/3.0/ 10.2174/1874210600903010080 1553505053 https://opendentistryjournal.com/VOLUME/3/PAGE/80/ https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-80/TODENTJ-3-80.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-80/TODENTJ-3-80.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-80/TODENTJ-3-80.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-80/TODENTJ-3-80.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-80/TODENTJ-3-80.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-80/TODENTJ-3-80.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-80/TODENTJ-3-80.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-80/TODENTJ-3-80.xml Dent Clin North Am Marsh PD 43 599 1999 Marsh PD. 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