TODENTJ-25-03-2019_TODENTJ-3-219 1553505108 Bentham Open doi@benthamopen.net Bentham Open The Open Dentistry Journal TODENTJ 1874-2106 11 5 2009 3 1 N Gagliano F Costa G.M Tartaglia L Pettinari F Grizzi C Sforza N Portinaro M Gioia G Annoni Background: We aimed at characterizing the aging gingiva analyzing: i) collagen content and turnover in human gingival tissues and fibroblasts obtained from healthy young and aging subjects. ii) the effect of cyclosporin A administration in human cultured gingival fibroblasts obtained from aging compared to young subjects. Methods: Morphological analysis was performed on haematoxylin-eosin and Sirius red stained paraffin-embedded gingival biopsies from young and aging healthy subjects. The expression of the main genes and proteins involved in collagen turnover were determined by real time PCR, dot blot and SDS-zymography on cultured young and aging gingival fibroblasts, and after cyclosporin A administration. Results: Our results suggest that in healthy aged people, gingival connective tissue is characterized by a similar collagen content and turnover. Collagen turnover pathways are similarly affected by cyclosporin A treatment in young and aging gingival fibroblasts. Conclusions: Cyclosporin A administration affects gingival collagen turnover pathways in young and aging fibroblasts at the same extent, suggesting that during aging cyclosporin A administration is not related to relevant collagen turnover modifications. 11 5 2009 219 226 TODENTJ-3-219 1 10.2174/crossmark_policy opendentistryjournal.com benthamopen.com true Peer Reviewed Single blind Checked with iThenticate 2008-12-30 2009-3-7 2009-8-18 2009-11-5 http://creativecommons.org/licenses/by-nc/3.0/ 10.2174/1874210600903010219 1553505108 https://opendentistryjournal.com/VOLUME/3/PAGE/219/ https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-219/TODENTJ-3-219.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-219/TODENTJ-3-219.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-219/TODENTJ-3-219.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-219/TODENTJ-3-219.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-219/TODENTJ-3-219.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-219/TODENTJ-3-219.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-219/TODENTJ-3-219.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-219/TODENTJ-3-219.xml JAGS Saunders MJ 49 1000 2001 Saunders MJ. 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