TODENTJ-25-03-2019_TODENTJ-3-208 1553505103 Bentham Open doi@benthamopen.net Bentham Open The Open Dentistry Journal TODENTJ 1874-2106 10 16 2009 3 1 P.R Schmidlin T Imfeld P Sahrmann A Tchouboukov F.E Weber Background and Objective: Povidone-iodine [polyvinylpyrrolidone-iodine complex (PVP-I)] is a broad-spectrum antimicrobial agent, frequently used in dentistry. In this study we investigated the short- and longterm effects on osteoblast number, viability, and function after short exposure to PVP-I with and without additional bone-morphogenetic protein-2 (BMP-2). Material and Methods: Confluent osteoblast-like cell line (MC3T3-E1, subclone 24) cultures were exposed to pure PVP-I solution (7.7 mg/ml) and dilutions of 1:10, 1:100 and 1:1000 for 10 seconds and washed with phosphate buffer solution. Cell proliferation and viability was determined by MTT and differentiation status by alkaline phosphatase (ALP) activity 6 days after initial plating. In a separate experiment, long-term cell proliferation, viability and function were assessed 4 weeks after PVP-I treatment by MTT and deposited calcium using an Alizarin-red staining test. Results: PVP-I decreased ALP activity substantially. Stimulation by BMP-2 recovered ALP activity to near control levels at 1:100 and 1:1000 dilutions of PVP-I. The MTT assay showed reduced proliferation of the preosteoblastic cells for all treatments, irrespective whether BMP-2 was used or not. Only at PVP-I dilutions of 1:1000 proliferation rate was back to normal levels (95.6±2.4 %). No adverse long-term effect of PVP-I on mineralization of the extracellular matrix (Alizarinred) for dilutions higher than 1:100 was observed. Interestingly, undiluted and 1:10 diluted PVP-I even showed a significant increase in mineral deposition, especially in the presence of BMP-2. Conclusion: Short-time application of PVP-I in concentrations of 1:10 and higher lead to decreased viability and impaired differentiation. However, surviving cells showed good recovery and mineralization potential. 10 16 2009 208 212 TODENTJ-3-208 1 10.2174/crossmark_policy opendentistryjournal.com benthamopen.com true Peer Reviewed Single blind Checked with iThenticate 2009-5-04 2009-6-03 2009-9-04 2009-10-16 http://creativecommons.org/licenses/by-nc/3.0/ 10.2174/1874210600903010208 1553505103 https://opendentistryjournal.com/VOLUME/3/PAGE/208/ https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-208/TODENTJ-3-208.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-208/TODENTJ-3-208.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-208/TODENTJ-3-208.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-208/TODENTJ-3-208.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-208/TODENTJ-3-208.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-208/TODENTJ-3-208.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-208/TODENTJ-3-208.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-208/TODENTJ-3-208.xml Periodontol 2000 Page RC 14 9 1997 Page RC, Kornman KS. The pathogenesis of human periodontitis: an introduction Periodontol 2000 1997; 14 : 9-11. J Periodontol Greenstein G 76 2015 2005 Greenstein G. Position paper: The role of supra- and subgingival irrigation in the treatment of periodontal diseases J Periodontol 2005; 76 : 2015-7. J Can Dent Assoc Bidault P 73 721 2007 Bidault P, Chandad F, Grenier D. Risk of bacterial resistance associated with systemic antibiotic therapy in periodontology J Can Dent Assoc 2007; 73 : 721-25. J Periodontal Res Slots J 37 389 2002 Slots J. Selection of antimicrobial agents in periodontal therapy J Periodontal Res 2002; 37 : 389-98. J Periodontol Greenstein G 70 1397 1999 Greenstein G. Povidone-iodine's effects and role in the management of periodontal diseases: a review J Periodontol 1999; 70 : 1397-405. J Clin Periodontol Rosling B 28 1023 2001 Rosling B, Hellstrom MK, Ramberg P, Socransky SS, Lindhe J. The use of PVP-iodine as an adjunct to non-surgical treatment of chronic periodontitis J Clin Periodontol 2001; 28 : 1023-31. J Periodontal Res Hoang T 38 311 2003 Hoang T, Jorgensen MG, Keim RG, Pattison AM, Slots J. Povidone-iodine as a periodontal pocket disinfectant J Periodontal Res 2003; 38 : 311-17. SADJ Wilken R 56 455 2001 Wilken R, Botha SJ, Grobler A, Germishuys PJ. In vitro cytotoxicity of chlorhexidine gluconate, benzydamine-HCl and povidone iodine mouthrinses on human gingival fibroblasts SADJ 2001; 56 : 455-60. Clin Oral Investig Cabral CT 11 155 2007 Cabral CT, Fernandes MH. In vitro comparison of chlorhexidine and povidone-iodine on the long-term proliferation and functional activity of human alveolar bone cells Clin Oral Investig 2007; 11 : 155-64. J Hosp Infect Gocke DJ 6(A) 59 1985 Gocke DJ, Ponticas S, Pollack W. In vitro studies of the killing of clinical isolates by povidone-iodine solutions J Hosp Infect 1985; 6(A) : 59-66. Dermatology Shiraishi T 204 37 2002 Shiraishi T, Nakagawa Y. Evaluation of the bactericidal activity of povidone-iodine and commercially available gargle preparations Dermatology 2002; 204 (1) : 37-41. Dermatology Yoneyama A 212 103 2006 Yoneyama A, Shimizu M, Tabata M, Yashiro J, Takata T, Hikida M. In vitro short-time killing activity of povidone-iodine (isodine gargle) in the presence of oral organic matter Dermatology 2006; 212 (1) : 103-8. Vet Surg Sanchez IR 17 291 1988 Sanchez IR, Swaim SF, Nusbaum KE, Hale AS, Henderson RA, McGuire JA. Effects of chlorhexidine diacetate and povidone-iodine on wound healing in dogs Vet Surg 1988; 17 : 291-95.