TODENTJ-25-03-2019_TODENTJ-3-191 1553505096 Bentham Open doi@benthamopen.net Bentham Open The Open Dentistry Journal TODENTJ 1874-2106 9 7 2009 3 1 Chieko Ishikawa Takumi Ogawa Tomoko Ikawa Akira Yamane We compared the hypertrophic effects of clenbuterol, a β2-adrenergic agonist, on the masseter, digastric, and temporalis with those on the tongue, tibialis anterior, soleus, diaphragm, and heart. The weights of masseter, digastric and temporalis in the clenbuterol group were 36 ~ 56% greater than those in the control group, whereas those of the tibialis anterior, diaphragm, and heart weights in the clenbuterol group were 9 ~ 33% greater than those in the control group. No significant difference in the weights of the soleus and tongue was found between the control and clenbuterol groups. Taken together with our present and previously reported results, it is suggested that the hypertrophic effects of clenbuterol on the masseter, digastric, and temporalis are greater than those on the limb, trunk, and heart. 9 7 2009 191 196 TODENTJ-3-191 1 10.2174/crossmark_policy opendentistryjournal.com benthamopen.com true Peer Reviewed Single blind Checked with iThenticate 2009-6-15 2009-7-2 2009-7-31 2009-9-7 http://creativecommons.org/licenses/by-nc/3.0/ 10.2174/1874210600903010191 1553505096 https://opendentistryjournal.com/VOLUME/3/PAGE/191/ https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-191/TODENTJ-3-191.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-191/TODENTJ-3-191.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-191/TODENTJ-3-191.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-191/TODENTJ-3-191.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-191/TODENTJ-3-191.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-191/TODENTJ-3-191.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-191/TODENTJ-3-191.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-191/TODENTJ-3-191.xml J Pharmacol Sci Sato S 107 393 2008 Sato S, Nomura S, Kawano F, Tanihata J, Tachiyashiki K, Imaizumi K. Effects of the β2-agonist clenbuterol on β1- and β2-adrenoceptor mRNA expressions of rat skeletal and left ventricle muscles J Pharmacol Sci 2008; 107 : 393-400. Biosci Reports Emery PW 4 83 1984 Emery PW, Rothwell NJ, Stock MJ, Winter PD. Chronic effects of β2-adrenergic agonists on body composition and protein synthesis in the rat Biosci Reports 1984; 4 : 83-91. J Surg Res Benson DW 50 1 1991 Benson DW, Foley-Nelson T, Chance WT, Zhang FS, James JH, Fischer JE. Decreased myofibrillar protein breakdown following treatment with clenbuterol J Surg Res 1991; 50 : 1-5. Am J Physiol Cell Physiol Stevens L 279 C1558 2000 Stevens L, Firinga C, Gohlsch B, Bastide B, Mounier Y, Pette D. Effects of unweighting and clenbuterol on myosin light and heavy chains in fast and slow muscles of rat Am J Physiol Cell Physiol 2000; 279 : C1558-63. Acta Physiol Scand Oishi Y 176 311 2002 Oishi Y, Imoto K, Ogata T, Taniguchi K, Matsumoto H, Roy RR. Clenbuterol induces expression of multiple myosin heavy chain isoforms in rat soleus fibres Acta Physiol Scand 2002; 176 : 311-18. Am J Physiol Regul Integr Comp Physiol Rajab P 279 R1076 2000 Rajab P, Fox J, Riaz S, Tomlinson D, Ball D, Greenhaff PL. Skeletal muscle myosin heavy chain isoforms and energy metabolism after clenbuterol treatment in the rat Am J Physiol Regul Integr Comp Physiol 2000; 279 : R1076-81. Jpn J Oral Biol Wakana N 45 418 2003 Wakana N, Akutsu S, Yamane A. Effects of clenbuterol, a β2-adrenergic agonist, on the myofiber diameter, fiber type, and expressions of insulin-like growth factors in the adult mouse masseter muscle Jpn J Oral Biol 2003; 45 : 418-27. Am J Physiol Heart Circ Physiol Soppa GK 288 H1468 2005 Soppa GK, Smolenski RT, Latif N, et al. Effects of chronic administration of clenbuterol on function and metabolism of adult rat cardiac muscle Am J Physiol Heart Circ Physiol 2005; 288 : H1468-76. Cardiovasc Res Wong K 37 115 1998 Wong K, Boheler KR, Bishop J, Petrou M, Yacoub MH. Clenbuterol induces cardiac hypertrophy with normal functional, morphological and molecular features Cardiovasc Res 1998; 37 : 115-22. Br J Pharmacol Akutsu S 147 412 2006 Akutsu S, Shimada A, Yamane A. Transforming growth factor βs are upregulated in the rat masseter muscle hypertrophied by clenbuterol, a β2 adrenergic agonist Br J Pharmacol 2006; 147 : 412-21. Am J Physiol Endocrinol Metab Awede BL 282 E31 2002 Awede BL, Thissen JP, Lebacq J. Role of IGF-I and IGFBPs in the changes of mass and phenotype induced in rat soleus muscle by clenbuterol Am J Physiol Endocrinol Metab 2002; 282 : E31-7. Am J Physiol Endocrinol Metab Sneddon AA 281 E676 2001 Sneddon AA, Delday MI, Steven J, Maltin CA. Elevated IGF-II mRNA and phosphorylation of 4E-BP1 and p70S6k in muscle showing clenbuterol-induced anabolism Am J Physiol Endocrinol Metab 2001; 281 : E676-82. Arch Oral Biol Matsumoto T 51 603 2006 Matsumoto T, Akutsu S, Wakana N, Morito M, Shimada A, Yamane A. The expressions of insulin-like growth factors, their receptors, and binding proteins are related to the mechanism regulating masseter muscle mass in the rat Arch Oral Biol 2006; 51 : 603-11. Muscle Nerve Hinkle RT 25 729 2002 Hinkle RT, Hodge KMB, Cody DB, Sheldon RJ, Kobilka BK, Isfort RJ. Skeletal muscle hypertrophy and anti-atrophy effects of clenbuterol are mediated by the β2-adrenergic receptor Muscle Nerve 2002; 25 : 729-34. J Appl Physiol Adams GR 96 1613 2004 Adams GR, Cheng DC, Haddad F, Baldwin KM. Skeletal muscle hypertrophy in response to isometric, lengthening, and shortening training bouts of equivalent duration J Appl Physiol 2004; 96 : 1613-8. Exerc Sport Sci Rev Adams GR 26 31 1998 Adams GR. Role of insulin-like growth factor-I in the regulation of skeletal muscle adaptation to increased loading Exerc Sport Sci Rev 1998; 26 : 31-60. Endocr Rev Jones JI 16 3 1995 Jones JI, Clemmons DR. Insulin-like growth factors and their binding proteins: Biological actions Endocr Rev 1995; 16 : 3-34. Endocr Rev Florini JR 17 481 1996 Florini JR, Ewton DZ, Coolican SA. Growth hormone and the insulin-like growth factor system in myogenesis Endocr Rev 1996; 17 : 481-517. Int J Dev Biol Yamane A 46 807 2002 Yamane A, Urushiyama T, Diekwisch TGH. Roles of insulin-like growth factors and their binding proteins in the differentiation of mouse tongue myoblasts Int J Dev Biol 2002; 46 : 807-16. Dev Growth Differ Yamane A 45 1 2003 Yamane A, Amano O, Slavkin HC. Insulin-like growth factors, hepatocyte growth factor and transforming growth factor-α in mouse tongue myogenesis Dev Growth Differ 2003; 45 : 1-6. Physiol Rev Charge SB 84 209 2004 Charge SB, Rudnicki MA. Cellular and molecular regulation of muscle regeneration Physiol Rev 2004; 84 : 209-38. J Appl Physiol Hawke TJ 91 534 2001 Hawke TJ, Garry DJ. Myogenic satellite cells: physiology to molecular biology J Appl Physiol 2001; 91 : 534-1. Clin Genet Sabourin LA 57 16 2000 Sabourin LA, Rudnicki MA. The molecular regulation of myogenesis Clin Genet 2000; 57 : 16-25. Am J Physiol Cell Physiol Yamane A 289 C42 2005 Yamane A, Akutsu S, Diekwisch TG, Matsuda R. Satellite cells and utrophin are not directly correlated with the degree of skeletal muscle damage in mdx mice Am J Physiol Cell Physiol 2005; 289 : C42-8. Muscle Nerve Burniston JG 33 655 2006 Burniston JG, Clark WA, Tan LB, Goldspink DF. Dose-dependent separation of the hypertrophic and myotoxic effects of the β2-adrenergic receptor agonist clenbuterol in rat striated muscles Muscle Nerve 2006; 33 : 655-3. Biochem J MacLennan PA 264 573 1989 MacLennan PA, Edwards RH. Effects of clenbuterol and propranolol on muscle mass: Evidence that clenbuterol stimulates muscle β-adrenoceptors to induce hypertrophy Biochem J 1989; 264 : 573-9. Chirality von Deutsch DA 12 637 2000 von Deutsch DA, Abukhalaf IK, Wineski LE, et al. β-agonist-induced alterations in organ weights and protein content: comparison of racemic clenbuterol and its enantiomers Chirality 2000; 12 : 637-48. Biosci Rep Rothwell NJ 7 933 1987 Rothwell NJ, Stock MJ. Effect of a selective β2-adrenergic agonist (clenbuterol) on energy balance and body composition in normal and protein deficient rats Biosci Rep 1987; 7 : 933-40. Eur J Appl Physiol Occup Physiol Criswell DS 74 391 1996 Criswell DS, Powers SK, Herb RA. Clenbuterol-induced fiber type transition in the soleus of adult rats Eur J Appl Physiol Occup Physiol 1996; 74 : 391-6. Med Sci Sports Exerc Dodd SL 28 669 1996 Dodd SL, Powers SK, Vrabas IS, Criswell D, Stetson S, Hussain R. Effects of clenbuterol on contractile and biochemical properties of skeletal muscle Med Sci Sports Exerc 1996; 28 : 669-76. J Appl Physiol Lynch GS 81 1610 1996 Lynch GS, Hayes A, Campbell SP, Williams DA. Effects of β2-agonist administration and exercise on contractile activation of skeletal muscle fibers J Appl Physiol 1996; 81 : 1610-8. Am J Physiol Endocrinol Metab Gilson H 297 E157 2009 Gilson H, Schakman O, Kalista S, Lause P, Tsuchida K, Thissen JP. Follistatin induces muscle hypertrophy through satellite cell proliferation and inhibition of both myostatin and activin Am J Physiol Endocrinol Metab 2009; 297 : E157-64. Cell Metab Serrano AL 7 33 2008 Serrano AL, Baeza-Raja B, Perdiguero E, Jardi M, Munoz-Canoves P. Interleukin-6 is an essential regulator of satellite cell-mediated skeletal muscle hypertrophy Cell Metab 2008; 7 : 33-44. J Anim Sci McMillan DN 70 3014 1992 McMillan DN, Noble BS, Maltin CA. The effect of the β-adrenergic agonist clenbuterol on growth and protein metabolism in rat muscle cell cultures J Anim Sci 1992; 70 : 3014-23. J Anat Roberts P 180(Pt 1) 57 1992 Roberts P, McGeachie JK. The effects of clenbuterol on satellite cell activation and the regeneration of skeletal muscle: an autoradiographic and morphometric study of whole muscle transplants in mice J Anat 1992; 180(Pt 1) : 57-65. Muscle Nerve Maltin CA 15 919 1992 Maltin CA, Delday MI. Satellite cells in innervated and denervated muscles treated with clenbuterol Muscle Nerve 1992; 15 : 919-25. Am J Anat Noden DM 168 257 1983 Noden DM. The embryonic origins of avian cephalic and cervical muscles and associated connective tissues Am J Anat 1983; 168 : 257-76. Anat Embryol Christ B 191 381 1995 Christ B, Ordahl CP. Early stages of chick somite development Anat Embryol 1995; 191 : 381-96. Anat Embryol (Berl) Huang R 200 137 1999 Huang R, Zhi Q, Izpisua-Belmonte JC, Christ B, Patel K. Origin and development of the avian tongue muscles Anat Embryol (Berl) 1999; 200 : 137-52. Cell Tissue Res Yamane A 322 183 2005 Yamane A. Embryonic and postnatal development of masticatory and tongue muscles Cell Tissue Res 2005; 322 : 183-89.