TODENTJ-25-03-2019_TODENTJ-3-120 1553505065 Bentham Open doi@benthamopen.net Bentham Open The Open Dentistry Journal TODENTJ 1874-2106 6 9 2009 3 1 Gloria Denotti Rosaria Piga Caterina Montaldo Matteo Erriu Francesca Pilia Alessandra Piras Massimo De Luca Germano Orrù E. faecalis in endodontic infection represents a biofilm type of disease, which explains the bacteria’s resistance to various antimicrobial compounds and the subsequent failure after endodontic treatment. The purpose of this study was to compare antimicrobial activities and bacteria kinetic adhesion in vitro for three endodontic medicaments with a clinical isolate of E. faecalis. We devised a shake culture which contained the following intracanalar preparations: CPD, Endoidrox (EIX), PulpCanalSealer (PCS); these were immersed in a liquid culture medium inoculated with the microorganism. The shake system velocity was able to prevent non-specific bacteria adhesion and simulated the salivary flow. Specimens were collected daily (from both the medium and medicaments) for 10 days; the viable cells were counted by plate count, while the adhesion index AI° [E. faecalis fg DNA] /mm2 was evaluated in the pastes after DNA extraction, by quantitative real time PCR for the 16S rRNA gene. A partial growth inhibition, during the first 24 hours, was observed in the liquid medium and on the medicaments for EIX and subsequently for CPD (six logs). EIX showed the lowest adhesion coefficient (5*102 [fg DNA]/mm2) for nine days and was similar to the control. PCS showed no antimicrobial/antibiofilm properties. This showed that “calcium oxide” base compounds could be active against biofilm progression and at least in the short term (2-4 days) on E. faecalis cells growing in planktonic cultures. 6 9 2009 120 124 TODENTJ-3-120 1 10.2174/crossmark_policy opendentistryjournal.com benthamopen.com true Peer Reviewed Single blind Checked with iThenticate 2008-10-14 2009-1-12 2009-4-3 2009-6-9 http://creativecommons.org/licenses/by-nc/3.0/ 10.2174/1874210600903010120 1553505065 https://opendentistryjournal.com/VOLUME/3/PAGE/120/ https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-120/TODENTJ-3-120.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-120/TODENTJ-3-120.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-120/TODENTJ-3-120.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-120/TODENTJ-3-120.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-120/TODENTJ-3-120.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-120/TODENTJ-3-120.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-120/TODENTJ-3-120.pdf https://opendentistryjournal.com/contents/volumes/V3/TODENTJ-3-120/TODENTJ-3-120.xml Tannock GW, Cook G. 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