TODENTJ-25-03-2019_TODENTJ-11-595 1553507214 Bentham Open doi@benthamopen.net Bentham Open The Open Dentistry Journal TODENTJ 1874-2106 11 24 2017 11 1 Amir Moeintaghavi Hamid Reza Arab Seyed Abdol Rahim Rezaee Hani Naderi Farid Shiezadeh Saber Sadeghi Najme Anvari Aim: The purpose of this study was to compare IL-1β and IL-12 gene expression in the gingival tissue of smokers and non-smokers either with healthy periodontium or with chronic periodontitis. Materials and Methods: 41 individuals consisting of 21 healthy controls (11 non-smokers and 10 smokers) and 20 chronic periodontitis patients (10 non-smokers and 10 smokers) participated in this study. Samples were collected from papillary regions of targeted areas and cytokines were analyzed using Real Time PCR. Shapiro-Wilk, Mann-Witney and Independent T tests were employed for statistical analysis. Results: IL-1β gene expression in gingival tissue of non-smoker group with chronic periodontitis was significantly higher than non-smoker-healthy group (p=0.011). Smoker-chronic periodontitis group showed lower IL-1β gene expression than non-smoker-chronic periodontitis group (p=0.003). IL-12 gene expression was not significantly different between analyzed groups. Conclusion: IL-1β gene expression increases in gingival tissue of non-smoker-chronic periodontitis patients due to inflammatory processes but smoking reduces the expression of this cytokine in diseased periodontal tissues. On the other hand periodontal condition and smoking habits do not seem to affect IL-12 gene expressions in gingival tissues. Authors concluded that reduced levels of IL1 and in some extent IL12 in smoking patients are responsible for higher tissue and bone degenerations and less treatment responses in smokers. 11 24 2017 595 602 TODENTJ-11-595 1 10.2174/crossmark_policy opendentistryjournal.com benthamopen.com true Peer Reviewed Single blind Checked with iThenticate 2017-06-04 2017-09-12 2017-10-18 2017-11-24 https://creativecommons.org/licenses/by/4.0/legalcode 10.2174/1874210601711010595 1553507214 https://opendentistryjournal.com/VOLUME/11/PAGE/595/ https://opendentistryjournal.com/contents/volumes/V11/TODENTJ-11-595/TODENTJ-11-595.pdf https://opendentistryjournal.com/contents/volumes/V11/TODENTJ-11-595/TODENTJ-11-595.pdf https://opendentistryjournal.com/contents/volumes/V11/TODENTJ-11-595/TODENTJ-11-595.pdf https://opendentistryjournal.com/contents/volumes/V11/TODENTJ-11-595/TODENTJ-11-595.pdf https://opendentistryjournal.com/contents/volumes/V11/TODENTJ-11-595/TODENTJ-11-595.pdf https://opendentistryjournal.com/contents/volumes/V11/TODENTJ-11-595/TODENTJ-11-595.pdf https://opendentistryjournal.com/contents/volumes/V11/TODENTJ-11-595/TODENTJ-11-595.pdf https://opendentistryjournal.com/contents/volumes/V11/TODENTJ-11-595/TODENTJ-11-595.xml 10.1111/j.1600-051X.1995.tb00256.x Martinez-Canut P, Lorca A, Magán R. 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