TODENTJ-25-03-2019_TODENTJ-10-308
1553506519
Bentham Open
doi@benthamopen.net
Bentham Open
The Open Dentistry Journal
TODENTJ
1874-2106
06
15
2016
10
1
Biological and Immunogenicity Property of IgY Anti S. mutans ComD
E.W.
Bachtiar
B.M.
Bachtiar
R.D.
Soejoedono
I.W.
Wibawan
A.
Afdhal
Objective: This study aims to elucidate the effect of IgY anti ComD on the biological properties of Streptococcus mutans. (S. mutans) ComD is an interspecies quorum-sensing signaling receptor that plays an important role in biofilm formation by S. mutans.
Materials and Methodology: Egg yolk IgY was produced by the immunization of chickens with a DNA vaccine containing the ComD DNA coding region. We evaluated the effect of the antibody on biofilm formation by S. mutans isolated from subjects with or without dental caries. We also assessed the immunoreactivity of the antibody against all isolates, and analyzed the protein profile of S. mutans by SDS-PAGE.
Results: The ComD antibody was successfully induced in the hens’ eggs. It inhibited biofilm formation by all S. mutans isolates. In addition, the expression of some protein bands was affected after exposure to the antibody.
Conclusion: IgY anti-S. mutans ComD reduces biofilm formation by this bacterium and alters the protein profile of S. mutans.
06
15
2016
308
314
TODENTJ-10-308
1
10.2174/crossmark_policy
opendentistryjournal.com
benthamopen.com
true
Peer Reviewed
Single blind
Checked with iThenticate
2016-01-24
2016-05-01
2016-05-09
2016-06-15
https://creativecommons.org/licenses/by/4.0/legalcode
10.2174/1874210601610010308
1553506519
https://opendentistryjournal.com/VOLUME/10/PAGE/308/
-
https://opendentistryjournal.com/contents/volumes/V10/TODENTJ-10-308/TODENTJ-10-308.pdf
-
https://opendentistryjournal.com/contents/volumes/V10/TODENTJ-10-308/TODENTJ-10-308.pdf
-
https://opendentistryjournal.com/contents/volumes/V10/TODENTJ-10-308/TODENTJ-10-308.pdf
-
https://opendentistryjournal.com/contents/volumes/V10/TODENTJ-10-308/TODENTJ-10-308.pdf
-
https://opendentistryjournal.com/contents/volumes/V10/TODENTJ-10-308/TODENTJ-10-308.pdf
-
https://opendentistryjournal.com/contents/volumes/V10/TODENTJ-10-308/TODENTJ-10-308.pdf
-
https://opendentistryjournal.com/contents/volumes/V10/TODENTJ-10-308/TODENTJ-10-308.pdf
-
https://opendentistryjournal.com/contents/volumes/V10/TODENTJ-10-308/TODENTJ-10-308.xml
Adv. Exp. Med. Biol.
Senadheera D.
631
178
2008
10.1007/978-0-387-78885-2_12
Senadheera D, Cvitkovitch DG.
Quorum sensing and biofilm formation by Streptococcus mutans.
Adv Exp Med Biol
2008;
631
: 178-88.
Biochem. Biophys. Res. Commun.
Zhang K.
379
933
2009
10.1016/j.bbrc.2008.12.175
Zhang K, Ou M, Wang W, Ling J.
Effects of quorum sensing on cell viability in Streptococcus mutans biofilm formation.
Biochem Biophys Res Commun
2009;
379
(4)
: 933-8.
J. Bacteriol.
Li Y.H.
184
2699
2002
10.1128/JB.184.10.2699-2708.2002
Li YH, Tang N, Aspiras MB, et al.
A quorum-sensing signaling system essential for genetic competence in Streptococcus mutans is involved in biofilm formation.
J Bacteriol
2002;
184
(10)
: 2699-708.
Sensors (Basel)
Li Y.H.
12
2519
2012
10.3390/s120302519
Li YH, Tian X.
Quorum sensing and bacterial social interactions in biofilms.
Sensors (Basel)
2012;
12
(3)
: 2519-38.
Infect. Immun.
Merritt J.
71
1972
2003
10.1128/IAI.71.4.1972-1979.2003
Merritt J, Qi F, Goodman SD, Anderson MH, Shi W.
Mutation of luxS affects biofilm formation in Streptococcus mutans.
Infect Immun
2003;
71
(4)
: 1972-9.
J. Bacteriol.
Loo C.Y.
182
1374
2000
10.1128/JB.182.5.1374-1382.2000
Loo CY, Corliss DA, Ganeshkumar N.
Streptococcus gordonii biofilm formation: identification of genes that code for biofilm phenotypes.
J Bacteriol
2000;
182
(5)
: 1374-82.
Nippon Saikingaku Zasshi
Yoshida A.
62
263
2007
10.3412/jsb.62.263
Yoshida A.
Molecular genetic analysis for oral biofilm formation.
Nippon Saikingaku Zasshi
2007;
62
(2)
: 263-9.
Appl. Environ. Microbiol.
Yoshida A.
71
2372
2005
10.1128/AEM.71.5.2372-2380.2005
Yoshida A, Ansai T, Takehara T, Kuramitsu HK.
LuxS-based signaling affects Streptococcus mutans biofilm formation.
Appl Environ Microbiol
2005;
71
(5)
: 2372-80.
J. Exp. Med.
Akbari O.
189
169
1999
10.1084/jem.189.1.169
Akbari O, Panjwani N, Garcia S, Tascon R, Lowrie D, Stockinger B.
DNA vaccination: transfection and activation of dendritic cells as key events for immunity.
J Exp Med
1999;
189
(1)
: 169-78.
Vaccine
André F.E.
21
593
2003
10.1016/S0264-410X(02)00702-8
André FE.
Vaccinology: past achievements, present roadblocks and future promises.
Vaccine
2003;
21
: 593-5.
Vaccine
Bazhan S.I.
22
1672
2004
10.1016/j.vaccine.2003.09.048
Bazhan SI, Belavin PA, Seregin SV, et al.
Designing and engineering of DNA-vaccine construction encoding multiple CTL-epitopes of major HIV-1 antigens.
Vaccine
2004;
22
(13-14)
: 1672-82.
FEMS Microbiol. Lett.
Bachtiar E.W.
227
211
2003
10.1016/S0378-1097(03)00683-9
Bachtiar EW, Sheng KC, Fifis T, et al.
Delivery of a heterologous antigen by a registered Salmonella vaccine (STM1).
FEMS Microbiol Lett
2003;
227
(2)
: 211-7.
Bachtiar EW.
Enhanchment of immune responses to Salmonella-delivered heterologous antigens.
Australia: RMIT University 2005.
Acta Microbiol. Immunol. Hung.
Bachtiar E.W.
56
403
2009
10.1556/AMicr.56.2009.4.10
Bachtiar EW, Coloe PJ, Smooker PM.
Construction and immunogenicity of Salmonella vaccine vector expressing HIV-1 antigen and MCP3.
Acta Microbiol Immunol Hung
2009;
56
(4)
: 403-15.
Oral Microbiol. Immunol.
Terao Y.
24
211
2009
10.1111/j.1399-302X.2008.00497.x
Terao Y, Isoda R, Murakami J, Hamada S, Kawabata S.
Molecular and biological characterization of gtf regulation-associated genes in Streptococcus mutans.
Oral Microbiol Immunol
2009;
24
(3)
: 211-7.
Hua Xi Kou Qiang Yi Xue Za Zhi
Yang D.
21
396
2003
Yang D, Liu T, Cao F.
An experimental study on PAc and GTF gene vaccines of Streptococcus mutans against rats caries: antibody levels in saliva and serum.
Hua Xi Kou Qiang Yi Xue Za Zhi
2003;
21
(5)
: 396-9.
Int. J. ClinPrev. Dent.
Bachtiar E.W.
11
137
2015
10.15236/ijcpd.2015.11.3.137
Bachtiar EW, Bachtiar BM.
Construction of Caries DNA Vaccine pCDNA-ComD.
Int J ClinPrev Dent
2015;
11
(3)
: 137-42.
J. Investig. Clin. Dent.
Bachtiar E.W.
6
197
2015
10.1111/jicd.12143
Bachtiar EW, Bachtiar BM, Dewiyani S, Surono Akbar SM.
Enterococcus faecalis with capsule polysaccharides type 2 and biofilm-forming capacity in Indonesians requiring endodontic treatment.
J Investig Clin Dent
2015;
6
(3)
: 197-205.
Microbiology
Kreth J.
153
1799
2007
10.1099/mic.0.2007/005975-0
Kreth J, Hung DC, Merritt J, et al.
The response regulator ComE in Streptococcus mutans functions both as a transcription activator of mutacin production and repressor of CSP biosynthesis.
Microbiology
2007;
153
(Pt 6)
: 1799-807.
J. Dent. Res.
Fan M.W.
81
784
2002
10.1177/154405910208101112
Fan MW, Bian Z, Peng ZX, et al.
A DNA vaccine encoding a cell-surface protein antigen of Streptococcus mutans protects gnotobiotic rats from caries.
J Dent Res
2002;
81
(11)
: 784-7.
Vaccine
Jia R.
22
2511
2004
10.1016/j.vaccine.2004.01.025
Jia R, Guo JH, Fan MW, et al.
Mucosal immunization against dental caries with plasmid DNA encoding pac gene of Streptococcus mutans in rats.
Vaccine
2004;
22
(20)
: 2511-6.
Front. Biosci.
Banas J.A.
9
1267
2004
10.2741/1305
Banas JA.
Virulence properties of Streptococcus mutans.
Front Biosci
2004;
9
: 1267-77.
Crit. Rev. Oral Biol. Med.
Banas J.A.
14
89
2003
10.1177/154411130301400203
Banas JA, Vickerman MM.
Glucan-binding proteins of the oral streptococci.
Crit Rev Oral Biol Med
2003;
14
(2)
: 89-99.
Infect. Immun.
Hazlett K.R.
66
2180
1998
Hazlett KR, Michalek SM, Banas JA.
Inactivation of the gbpA gene of Streptococcus mutans increases virulence and promotes in vivo accumulation of recombinations between the glucosyltransferase B and C genes.
Infect Immun
1998;
66
(5)
: 2180-5.
Int. J. Oral Sci.
Decker E.M.
6
195
2014
10.1038/ijos.2014.38
Decker EM, Klein C, Schwindt D, von Ohle C.
Metabolic activity of Streptococcus mutans biofilms and gene expression during exposure to xylitol and sucrose.
Int J Oral Sci
2014;
6
(4)
: 195-204.